P4HB 蛋白, Mouse (HEK293, His)

P4HB proteins are versatile and multifunctional, coordinating disulfide bond catalysis at the cell surface and inside the cell.It exhibits reductase activity, cleaves disulfide bonds, induces structural modifications, and functions as a chaperone to inhibit aggregation of misfolded proteins.P4HB Protein, Mouse (HEK293, His) is the recombinant mouse-derived P4HB protein, expressed by HEK293 , with C-His labeled tag.

P4HB 蛋白是一种多功能实体，协调二硫键形成、断裂和重排的催化作用。它位于细胞表面，具有还原酶活性，可裂解附着在细胞上的蛋白质的二硫键，并可能诱导外表面蛋白质的结构修饰。在细胞内部，P4HB 在新生蛋白质中二硫键的形成和重排中发挥作用。在升高的浓度下，并在被 FAM20C 磷酸化后，它转变为伴侣，抑制错误折叠蛋白质的聚集，而在较低的浓度下，它促进聚集，表现出抗伴侣活性。此外，P4HB 可能与其他伴侣合作，在激素生物发生过程中改变甲状腺球蛋白前体的结构。此外，它还可作为多种酶的结构亚基，包括脯氨酰 4-羟化酶和微粒体三酰甘油转移蛋白 MTTP。值得注意的是，P4HB 还充当 LGALS9 的受体，这种相互作用影响其在细胞表面的定位，增强二硫键还原酶活性，改变质膜氧化还原状态，并促进细胞迁移。

Measured by its ability to promote aggregation of insulin in the presence of DTT. The specific activity is > 10 A₆₅₀/cm/min/mg.

Mouse

HEK293

C-His

P09103 (D20-L509)

18453  [NCBI]

DALEEEDNVLVLKKSNFEEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAAKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFKNGDTASPKEYTAGREADDIVNWLKKRTGPAATTLSDTAAAESLVDSSEVTVIGFFKDVESDSAKQFLLAAEAIDDIPFGITSNSGVFSKYQLDKDGVVLFKKFDEGRNNFEGEITKEKLLDFIKHNQLPLVIEFTEQTAPKIFGGEIKTHILLFLPKSVSDYDGKLSSFKRAAEGFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESDELTAEKITEFCHRFLEGKIKPHLMSQEVPEDWDKQPVKVLVGANFEEVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIIIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQDGAGDDEDLDLEEALEPDMEEDDDQKAVKDEL

57-70 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.