1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. PCSK1 蛋白, Human (HEK293, His)

PCSK1 蛋白, Human (HEK293, His)

目录号: HY-P77462
COA 产品使用指南

PCSK1 蛋白是一种前蛋白转化酶,在具有碱性氨基酸残基的位点处理激素和蛋白质前体方面发挥着关键作用。它针对多种底物,如 POMC、肾素、脑啡肽、强啡肽、生长抑素、胰岛素和 AGRP。PCSK1 蛋白, Human (HEK293, His) 是重组的 PCSK1 蛋白,由 HEK293 表达,带有 C-His 标签。

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The PCSK1 protein is a proprotein convertase that plays a key role in processing hormone and protein precursors at sites with basic amino acid residues. It targets multiple substrates such as POMC, renin, enkephalin, dynorphin, somatostatin, insulin and AGRP. PCSK1 Protein, Human (HEK293, His) is the recombinant human-derived PCSK1 protein, expressed by HEK293 , with C-His labeled tag.

研究背景

PCSK1 蛋白在激素和其他蛋白质前体的加工中发挥着关键作用,特别是在以成对碱性氨基酸残基为特征的位点上。作为前蛋白转化酶,PCSK1 靶向一系列底物,包括 POMC、肾素、脑啡肽、强啡肽、生长抑素、胰岛素和 AGRP。通过其酶活性,PCSK1 参与这些前体分子的蛋白水解裂解和成熟,从而调节活性激素和神经肽的生成。不同的底物凸显了 PCSK1 对对各种生理功能至关重要的蛋白质加工的广泛影响,包括新陈代谢、内分泌信号传导和神经肽调节。

生物活性

Measured by its ability to cleave the fluorogenic peptide 200 µM substrate pERTKR-AMC. that incubate at 37°C temperature for 60 minutes. The specific activity is 116.38 pmol/min/µg.

种属

Human

表达系统

HEK293

标签

C-10*His

蛋白编号

P29120-1/NP_000430.3 (S111-R617)

基因 ID
蛋白结构
N-term
PCSK1 (S111-R617)
Accession # P29120-1/NP_000430.3
His
C-term
同用名
Neuroendocrine convertase 1; Prohormone convertase 1; PC1; NEC1
氨基酸序列

SALRDSALNLFNDPMWNQQWYLQDTRMTAALPKLDLHVIPVWQKGITGKGVVITVLDDGLEWNHTDIYANYDPEASYDFNDNDHDPFPRYDPTNENKHGTRCAGEIAMQANNHKCGVGVAYNSKVGGIRMLDGIVTDAIEASSIGFNPGHVDIYSASWGPNDDGKTVEGPGRLAQKAFEYGVKQGRQGKGSIFVWASGNGGRQGDNCDCDGYTDSIYTISISSASQQGLSPWYAEKCSSTLATSYSSGDYTDQRITSADLHNDCTETHTGTSASAPLAAGIFALALEANPNLTWRDMQHLVVWTSEYDPLANNPGWKKNGAGLMVNSRFGFGLLNAKALVDLADPRTWRSVPEKKECVVKDNDFEPRALKANGEVIIEIPTRACEGQENAIKSLEHVQFEATIEYSRRGDLHVTLTSAAGTSTVLLAERERDTSPNGFKNWDFMSVHTWGENPIGTWTLRITDMSGRIQNEGRIVNWKLILHGTSSQPEHMKQPRVYTSYNTVQNDR

分子量

Approximately 60-70 kDa due to the glycosylation.

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末

组分

Lyophilized from a 0.22 μm filtered solution of 25 mM Tris-HCL, 150 mM NaCl, pH 7.5.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

PCSK1 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PCSK1 Protein, Human (HEK293, His)
目录号:
HY-P77462
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