PCSK9 蛋白, Rat (HEK293, His)

The PCSK9 protein regulates cholesterol levels by interacting with LDLR, VLDLR, LRP1/APOER, and LRP8/APOER2. It promotes their degradation and inhibits their recycling, leading to enhanced LDLR degradation. PCSK9 also affects APOB degradation, BACE1 intermediates, ENaC surface expression, and neuronal apoptosis via LRP8/APOER2. PCSK9 Protein, Rat (HEK293, His) is the recombinant rat-derived PCSK9 protein, expressed by HEK293 , with C-6*His labeled tag.

PCSK9 蛋白在血浆胆固醇稳态的调节中发挥着关键作用，通过与低密度脂蛋白受体家族成员的相互作用发挥其影响，包括低密度脂蛋白受体 (LDLR)、极低密度脂蛋白受体 (VLDLR) 、载脂蛋白 E 受体 (LRP1/APOER) 和载脂蛋白受体 2 (LRP8/APOER2)。PCSK9 促进细胞内酸性区室中这些受体的降解，采用非蛋白水解机制通过网格蛋白 LDLRAP1/ARH 介导的途径增强肝脏 LDLR 降解。此外，它可能阻碍 LDLR 从内体循环到细胞表面，引导其进入溶酶体降解，并诱导 LDLR 泛素化，导致随后的降解。值得注意的是，PCSK9 将其调节影响扩展到 LDLR 之外，独立于 LDLR 抑制 APOB 的细胞内降解，并参与早期分泌途径中 BACE1 非乙酰化中间体的处理。此外，PCSK9 的影响延伸至上皮 Na(+) 通道 (ENaC) 介导的 Na(+) 吸收，主要通过增加蛋白酶体降解来减少 ENaC 表面表达。该蛋白还通过调节 LRP8/APOER2 水平和相关的抗凋亡信号通路来调节神经元凋亡。

1.The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
2.Measured by its binding ability in a functional ELISA. When Recombinant Human LDLR is coated at 5 µg/mL can bind Recombinant Rat PCSK9. The ED₅₀ for this effect is 0.2335 μg/mL.

Rat

HEK293

C-6*His

P59996 (Q31-Q691)

298296  [NCBI]

QDEDGDYEELMLALPSQEDSLVDEASHVATATFRRCSKEAWRLPGTYVVVLMEETQRLQVEQTAHRLQTWAARRGYVIKVLHVFYDLFPGFLVKMSSDLLGLALKLPHVEYIEEDSLVFAQSIPWNLRIIPAWQQTEEDSSPDGSSQVEVYLLDTSIQSGHREIEGRVTITDFNSVPEEDGTRFHRQASKCDSHGTHLAGVVSGRDAGVAKGTSLHSLRVLNCQGKGTVSGTLIGLEFIRKSQLIQPSGPLVVLPLAGGYSRILNTACQRLARTGVVLVAAAGNFRDDACLYSPASAPEVITVGATNAQDQPVTLGTLGTNFGRCVDLFAPGKDIIGASSDCSTCYMSQSGTSQAAAHVAGIVAMMLNRDPALTLAELRQRILFSTKDVINMAWFPEDQRVLTPNRVATLPPSTQETGGQLLCRTVWSAHSGPTRTATATARCAPEEELLSCSSFSRSGRRRGDRIEAIGGQQVCKALNAFGGEGVYAVARCCLLPRVNCSIHNTPAAAGPQTPVHCHQKDHVLTGCSFHWEVENLRAQQQPLLRSRHQPGQCVGHQEASVHASCCHAPGLECKIKEHGIAGPAEQVTVACEAGWTLTGCNVLPGASLPLGAYSVDNVCVARIRDAGRADRTSEETVAAAICCRSRPSAKASWVHQ

Approximately 17.26 & 60.16kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.8.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.