PD-1 蛋白, Human (Biotinylated, HEK293, Fc-Avi)

PD-1 protein is an inhibitory receptor on T cells that maintains immune tolerance by binding to CD274/PDCD1L1 and CD273/PDCD1LG2. It blocks T cell activation by binding to CD3-TCR and recruiting PTPN11/SHP-2 to dephosphorylate key signaling molecules. PD-1 Protein, Human (Biotinylated, HEK293, Fc-Avi) is the recombinant human-derived PD-1 protein, expressed by HEK293 , with C-Avi, C-hFc labeled tag. The total length of PD-1 Protein, Human (Biotinylated, HEK293, Fc-Avi) is 143 a.a., with molecular weight of 65-72 kDa.

PD-1蛋白作为抗原激活T细胞的抑制性受体，在诱导和维持自身免疫耐受中发挥着至关重要的作用。PD-1 与其配体 CD274/PDCD1L1 和 CD273/PDCD1LG2 结合后，会传递抑制信号并与免疫突触中的 CD3-TCR 结合，直接阻碍 T 细胞激活。这种抑制作用通过招募 PTPN11/SHP-2 进一步执行，导致关键 TCR 近端信号分子去磷酸化。PD-1 与 CD274/PDCD1L1 的相互作用会被肿瘤利用来减弱抗肿瘤免疫力，从而抑制细胞毒性 T 淋巴细胞 (CTL) 效应器功能。阻断 PD-1 介导的通路有望逆转耗尽的 T 细胞表型并使抗肿瘤反应正常化，从而为癌症免疫治疗提供了理论依据。

Measured by its binding ability in a functional ELISA. When immobilized Human PD-L1 mFc tag at 2 μg/ml (100 μl/Well), can bind Biotinylated Human PD-1 hFc tag and the EC₅₀ is 0.35 μg/mL.

Human

HEK293

C-Avi;C-hFc

Q15116 (L25-Q167)

5133  [NCBI]

65-72 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.