PD-1 蛋白, Mouse (HEK293, Fc)

The PD-1 protein is expressed on antigen-activated T cells and can induce and maintain immune tolerance to itself.PD-1 binds to CD274/PDCD1L1 and CD273/PDCD1LG2, transmits inhibitory signals, inhibits T cell activation and regulates immune responses.PD-1 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived PD-1 protein, expressed by HEK293 , with C-hFc labeled tag.

PD-1 是抗原激活 T 细胞上表达的抑制性受体，在诱导和维持自身免疫耐受中发挥着至关重要的作用。在与 CD274/PDCD1L1 和 CD273/PDCD1LG2 等配体结合后，PD-1 会传递抑制信号，抑制 T 细胞活化并有助于免疫反应的调节。T 细胞受体结合后，PD-1 与免疫突触中的 CD3-TCR 结合，直接抑制 T 细胞激活。抑制作用涉及招募 PTPN11/SHP-2，使 TCR 附近的关键信号分子去磷酸化。PD-1介导的抑制途径被肿瘤利用，可减弱抗肿瘤免疫力并促进肿瘤存活。PD-1 以单体形式存在，与 CD274/PDCD1L1 和 CD273/PDCD1LG2 相互作用，而与 FBXO38 相互作用导致泛素化和蛋白酶体降解。

1. Determined by its ability to prevent plate adhesion of PHA-stimulated Jurkat cells in the presence of 625 ng/mL of bound hPD-L1. The ED50 this effect is 1.974 μg/mL, corresponding to a specific activity is 5.07×10^2 units/mg.
2. Immobilized Recombinant Mouse PD-1 at 1 μg/ml (100 μl/well) can bind Recombinant Mouse PD-L1. The ED₅₀ for this effect is 0.6932 μg/mL.

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  Determined by its ability to prevent plate adhesion of PHA-stimulated Jurkat cells in the presence of 625ng/mL of bound hPD-L1.  The ED₅₀ for this effect is 1.974 μg/mL, corresponding to a specific activity is 5.07×10² units/mg.

Mouse

HEK293

C-hFc

Q02242 (L25-Q167)

18566  [NCBI]

LEVPNGPWRSLTFYPAWLTVSEGANATFTCSLSNWSEDLMLNWNRLSPSNQTEKQAAFCNGLSQPVQDARFQIIQLPNRHDFHMNILDTRRNDSGIYLCGAISLHPKAKIEESPGAELVVTERILETSTRYPSPSPKPEGRFQ

Approximately 61.66 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.