PD-L1 蛋白, Rat (HEK293, Fc)

PD-L1 (Programmed death-ligand 1) critically regulates T cell proliferation and migration, acting as a biomarker for periodontitis and pre-eclampsia. CD274 is its human ortholog. Biased expression in the thymus (RPKM 102.9), spleen (RPKM 58.3), and other tissues emphasizes PD-L1's centrality in immune regulation across diverse physiological and pathological conditions. PD-L1 Protein, Rat (HEK293, Fc) is the recombinant rat-derived PD-L1 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of PD-L1 Protein, Rat (HEK293, Fc) is 221 a.a., with molecular weight of ~63 kDa.

程序性死亡配体 1 (PD-L1) 在 T 细胞增殖和细胞迁移的正向调节中发挥着关键作用。PD-L1 位于细胞表面，可作为牙周炎和先兆子痫等疾病的生物标志物。它在人类中的直系同源对应物是 CD274（CD274 分子）。PD-L1 在胸腺 (RPKM 102.9)、脾脏 (RPKM 58.3) 和各种其他组织中观察到偏向表达，是免疫调节的核心，在不同的生理和病理条件下具有重要意义。

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL‑2 mouse cytotoxic T cells. The ED ₅₀ this effect is 0.2157 μg/mL in the presence of 10 μg/mL anti-CD3, corresponding to a specific activity is 4.64×10³ units/mg.

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  Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL‑2 mouse cytotoxic T cells. The ED₅₀ for this effect is 0.2157 μg/mL in the presence of 10 μg/mL anti-CD3, corresponding to a specific activity is 4.64×10³ units/mg.

Rat

HEK293

C-hFc

NP_001178883.1 (A18-T238)

499342  [NCBI]

AFTITAPKDLYVVEYGSNVTMECRFPVEQKLDLLALVVYWEKEDKEVIQFVEGEEDLKPQHSSFRGRAFLPKDQLLKGNAVLQITDVKLQDAGVYCCMISYGGADYKRITLKVNAPYRKINQRISMDPATSEHELMCQAEGYPEAEVIWTNSDHQSLSGETTVTTSQTEEKLLNVTSVLRVNATANDVFHCTFWRVHSGENHTAELIIPELPVPRLPHNRT

Approximately 63 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.