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  3. PDE2A 蛋白, Human (sf9, His)

PDE2A 蛋白, Human (sf9, His)

目录号: HY-P74647
产品使用指南

PDE2A 是一种 cGMP 激活的环核苷酸磷酸二酯酶,对第二信使 cAMP 和 cGMP(各种生理过程中的关键调节因子)表现出双重特异性。PDE2A 通过覆盖 cAMP 表现出更高的效率,并在细胞生长和迁移中发挥重要作用。PDE2A 蛋白, Human (sf9, His) 是重组的 PDE2A 蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。PDE2A 蛋白, Human (sf9, His) 全长 686 个氨基酸,分子量约为 ~80.73 kDa。

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描述

PDE2A is a cGMP-activated cyclic nucleotide phosphodiesterase that exhibits dual specificity for the second messengers cAMP and cGMP, key regulators in various physiological processes. PDE2A exhibits higher efficiency by covering cAMP and plays an important role in cell growth and migration. PDE2A Protein, Human (sf9, His) is the recombinant human-derived PDE2A protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of PDE2A Protein, Human (sf9, His) is 686 a.a., with molecular weight of ~80.73 kDa.

研究背景

PDE2A 是一种 cGMP 激活的环核苷酸磷酸二酯酶,对第二信使 cAMP 和 cGMP(各种生理过程中的关键调节因子)表现出双重特异性。PDE2A 与 cAMP 相比,cGMP 具有更高的效率,在细胞生长和迁移中发挥着重要作用。此外,它还积极参与线粒体 cAMP 水平和呼吸的调节,影响线粒体的形态和动力学。值得注意的是,PDE2A 通过局部调节线粒体内的 cAMP/PKA 信号传导,复杂地参与细胞凋亡。这包括监测线粒体外膜的局部 cAMP 水平,并监督 DNM1L 的 PKA 依赖性磷酸化,强调其对细胞功能的多方面贡献。

种属

Human

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

O00408 (E215-H900)

基因 ID
蛋白结构
N-term
PDE2A (E215-H900)
Accession # O00408
His
C-term
同用名
cGMP-dependent 3',5'-cyclic phosphodiesterase; CGS-PDE; PDE2A
分子量

Approximately 80.73 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PDE2A Protein, Human (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PDE2A Protein, Human (sf9, His)
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