1. 重组蛋白
  2. Cytokines and Growth Factors
  3. PDGFs & PDGFRs
  4. PDGF
  5. PDGF-BB
  6. PDGF-BB 蛋白, Mouse

PDGF-BB 蛋白, Mouse

目录号: HY-P7087
COA 产品使用指南

PDGF-BB 蛋白, Mouse是一种有活性的 PDGF 亚型,可用于细胞培养。

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规格 价格 是否有货 数量
Free Sample   Apply now  
2 μg ¥750 In-stock
10 μg ¥1900 In-stock
50 μg ¥5900 In-stock
100 μg ¥9500 In-stock
> 100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

PDGF-BB Protein, Mouse is an active PDGF isoform, used in cell culture applications.

研究背景

血小板源性生长因子-BB (PDGF-BB) 主要由血小板 α 颗粒分泌,是骨骼和其他结缔组织中最活跃的 PDGF 亚型,因为它可以与所有已知的 PDGF 受体结合。PDGF-BB 通过诱导有丝分裂、趋化性、细胞外基质形成和血管化在骨再生中发挥重要作用[1]

生物活性

The ED50 is <2.5 ng/mL as measured by 3T3 cells, corresponding to a specific activity of >4 × 105 units/mg.

种属

Mouse

表达系统

E. coli

标签

Tag Free

蛋白编号

P31240 (S82-T190)

基因 ID
蛋白结构
N-term
PDGF-BB (S82-T190)
Accession # P31240
C-term
中文名
重组小鼠血小板衍生生长因子-BB
同用名
rMuPDGF-BB; PDGF-2; GDGF; ODGF; SIS; SSV
氨基酸序列

SLGSLAAAEPAVIAECKTRTEVFQISRNLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQCRASQVQMRPVQVRKIEIVRKKPIFKKATVTLEDHLACKCETIVTPRPVT

分子量

Approximately 13 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized after extensive dialysis against 10 mM Sodium Citrate, pH 3.0 or 20 mM Sodium Citrate, pH 4.0, 8% trehalose.

内毒素含量

<0.2 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

PDGF-BB Protein, Mouse 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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