PDGF-CC 蛋白, Mouse (HEK293, Fc)

The PDGF-CC protein is a key growth factor that plays crucial roles in a variety of cellular processes, including embryonic development, cell proliferation, migration, survival, and chemotaxis. It has potent mitogenic and chemoattractant properties and is essential during embryonic skeletal formation, craniofacial development, palate development, skin morphogenesis and wound healing stages. PDGF-CC Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived PDGF-CC protein, expressed by HEK293 , with N-hFc labeled tag. The total length of PDGF-CC Protein, Mouse (HEK293, Fc) is 111 a.a., with molecular weight of ~46 kDa.

PDGF-CC 蛋白是一种关键的生长因子，在协调不同的细胞过程（涵盖胚胎发育、细胞增殖、迁移、存活和趋化性）方面发挥着关键作用。PDGF-CC 表现出对间充质细胞有效的有丝分裂和化学引诱特性，在胚胎骨骼形成的复杂景观中发挥着关键作用，特别是在颅面和腭发育以及皮肤的形态发生中。它参与伤口愈合，包括对炎症、增殖和重塑阶段的关键贡献。此外，PDGF-CC 是血管生成和血管发育的核心人物，通过协调间质成纤维细胞向肌成纤维细胞的转化并促进胶原沉积，对纤维化过程产生影响。除了其典型作用之外，CUB 结构域还暗示着额外的促有丝分裂活性，特别是在冠状动脉平滑肌细胞中。在细胞核中，PDGF-CC 揭示了额外的功能，强调了其多方面的调节能力。同二聚体和二硫键连接的 PDGF-CC 与 PDGFRA 同二聚体和 PDGFRA 和 PDGFRB 形成的异二聚体发生复杂的相互作用，同时还通过其 CUB 结构域与 PLAT 相互作用。

Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblasts cells. The ED₅₀ this effect is 61.4 ng/mL, corresponding to a specific activity is 1.6287×10^4 units/mg.

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  Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblasts cells. The ED₅₀ for this effect is 61.4 ng/mL, corresponding to a specific activity is 1.6287×10⁴ units/mg.

Mouse

HEK293

N-hFc

Q8CI19 (V235-G345)

54635  [NCBI]

VVNLNLLKEEVKLYSCTPRNFSVSIREELKRTDTIFWPGCLLVKRCGGNCACCLHNCNECQCVPRKVTKKYHEVLQLRPKTGVKGLHKSLTDVALEHHEECDCVCRGNAGG

Approximately 46 kDa

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  Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.