PDGF R alpha 蛋白, Mouse (HEK293, His)

PDGF R α is a tyrosine-protein kinase receptor with critical effects on embryonic development, cell proliferation, survival, and chemotaxis.Its effects on cell behavior vary depending on the environment and is critical for differentiation of bone marrow mesenchymal stem cells, normal skeletal development, embryonic skull closure, and gastrointestinal mucosal formation.PDGF R alpha Protein, Mouse (HEK293, His) is the recombinant mouse-derived PDGF R alpha protein, expressed by HEK293 , with C-6*His labeled tag.

PDGF R α 蛋白是一种酪氨酸蛋白激酶，充当 PDGFA、PDGFB 和 PDGFC 的细胞表面受体。它在胚胎发育、细胞增殖、存活和趋化性中起着至关重要的作用。它对细胞增殖和迁移的影响因环境而异。该蛋白还参与骨髓间充质干细胞的分化，并且是胚胎发育过程中正常骨骼发育和头颅闭合所必需的。此外，它对于胃肠道内壁的发育、间充质细胞的募集以及肠绒毛的正常发育至关重要。该蛋白有助于伤口愈合中的细胞迁移和趋化性，并在血小板活化、血小板颗粒分泌激动剂以及凝血酶诱导的血小板聚集中发挥作用。蛋白质在与其配体结合时发生激活，配体可以是同二聚体PDGFA、同二聚体PDGFB、由PDGFA和PDGFB形成的异二聚体、或同二聚体PDGFC。这种激活导致多个信号级联的激活，响应取决于特定配体，并通过 PDGFRA 和 PDGFRB 之间异二聚体的形成进行调节。该蛋白磷酸化 PIK3R1、PLCG1 和 PTPN11，导致各种细胞反应，例如二酰甘油和肌醇 1,4,5-三磷酸的产生、胞质 Ca(2+) 的动员、蛋白激酶 C 的激活以及AKT1 信号通路。它还介导 HRAS 和 MAP 激酶 MAPK1/ERK2 和/或 MAPK3/ERK1 的激活。此外，它还促进 STAT 家族成员 STAT1、STAT3 和 STAT5A 和/或 STAT5B 的激活。受体信号传导受到使受体及其下游效应子去磷酸化的蛋白磷酸酶以及激活受体的快速内化的调节。

Mouse

HEK293

C-6*His

P26618 (L25-E524)

18595  [NCBI]

LLLPSILPNENEKIVQLNSSFSLRCVGESEVSWQHPMSEEDDPNVEIRSEENNSGLFVTVLEVVNASAAHTGWYTCYYNHTQTDESEIEGRHIYIYVPDPDMAFVPLGMTDSLVIVEEDDSAIIPCRTTDPETQVTLHNNGRLVPASYDSRQGFNGTFSVGPYICEATVKGRTFKTSEFNVYALKATSELNLEMDARQTVYKAGETIVVTCAVFNNEVVDLQWTYPGEVRNKGITMLEEIKLPSIKLVYTLTVPKATVKDSGEYECAARQATKEVKEMKRVTISVHEKGFVEIEPTFGQLEAVNLHEVREFVVEVQAYPTPRISWLKDNLTLIENLTEITTDVQKSQETRYQSKLKLIRAKEEDSGHYTIIVQNEDDVKSYTFELSTLVPASILDLVDDHHGSGGGQTVRCTAEGTPLPEIDWMICKHIKKCNNDTSWTVLASNVSNIITELPRRGRSTVEGRVSFAKVEETIAVRCLAKNNLSVVARELKLVAPTLRSE

80-120 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.