2. 重组蛋白
  3. Cytokines and Growth Factors
  4. PDGFs & PDGFRs
  5. PDGF
  6. PDGF-A
  7. PDGFAA 蛋白, Canine (HEK293, Fc)

PDGFAA 蛋白, Canine (HEK293, Fc)

目录号: HY-P75964
产品使用指南

PDGFAA 蛋白质是血小板衍生生长因子亚单位 A 前体。PDGFAA 蛋白, Canine (HEK293, Fc) 是重组的 PDGFAA 蛋白，由 HEK293 表达，带有 N-hFc 标签。PDGFAA 蛋白, Canine (HEK293, Fc) 全长 110 个氨基酸，分子量约为 ~41 kDa。

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描述

PDGFAA Protein is platelet-derived growth factor subunit A precursor. PDGFAA Protein, Canine (HEK293, Fc) is the recombinant canine-derived PDGFAA protein, expressed by HEK293 , with N-hFc labeled tag. The total length of PDGFAA Protein, Canine (HEK293, Fc) is 110 a.a., with molecular weight of ~41 kDa.
研究背景

PDGFAA 蛋白质是血小板衍生生长因子亚单位 A 前体。PDGFAA 使生长因子活性和血小板衍生生长因子受体结合成为可能。PDGFAA 参与血小板源性生长因子受体信号通路、MAP 激酶活性的正调节及磷脂酰肌醇 3-激酶/蛋白激酶 B 信号转导的正调节[1][2][3]。
种属

Canine
表达系统

HEK293
标签

N-hFc
蛋白编号

NP_001177101 (S87-R196)
基因 ID
蛋白结构
N-term
hFc
PDGFAA (S87-R196)
Accession # NP_001177101
C-term
同用名
Platelet-derived growth factor subunit A; PDGF-1; PDGF-A; Rpa1
分子量

Approximately 41 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

PDGFAA Protein, Canine (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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