2. 重组蛋白
  3. Enzymes & Regulators
  4. Oxidoreductases (EC 1)
  5. Peroxiredoxin-2/PRDX2 蛋白, Human (His)

Peroxiredoxin-2/PRDX2 蛋白, Human (His)

目录号: HY-P71464
产品使用指南

Peroxiredoxin-2 (PRDX2) 是一种硫醇特异性过氧化物酶，可催化过氧化氢和有机氢过氧化物的还原，这对于细胞保护免受氧化应激至关重要。它解毒过氧化物，感知过氧化氢介导的信号事件，并可能参与由生长因子和肿瘤坏死因子-α引发的信号级联。Peroxiredoxin-2/PRDX2 蛋白, Human (His) 是重组的 Peroxiredoxin-2/PRDX2 蛋白，由 E. coli 表达，带有 N-His 标签。Peroxiredoxin-2/PRDX2 蛋白, Human (His) 全长 197 个氨基酸，分子量约为 ~25.8 kDa。

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描述

Peroxiredoxin-2 (PRDX2) is a thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides, which is essential for cellular protection against oxidative stress. It detoxifies peroxide, senses hydrogen peroxide-mediated signaling events, and may participate in signaling cascades initiated by growth factors and tumor necrosis factor-alpha. Peroxiredoxin-2/PRDX2 Protein, Human (His) is the recombinant human-derived Peroxiredoxin-2/PRDX2 protein, expressed by E. coli , with N-His labeled tag. The total length of Peroxiredoxin-2/PRDX2 Protein, Human (His) is 197 a.a., with molecular weight of ~25.8 kDa.
研究背景

Peroxiredoxin-2 (PRDX2) 是一种硫醇特异性过氧化物酶，可作为还原过氧化氢和有机氢过氧化物的催化剂，将它们分别转化为水和醇。它在细胞保护免受氧化应激方面的重要作用涉及对过氧化物进行解毒并充当过氧化氢介导的信号事件的传感器。PRDX2 还可能参与由生长因子和肿瘤坏死因子-α 启动的信号级联反应，从而可能影响细胞内 H(2)O(2) 浓度。这种多功能蛋白质强调了其在细胞氧化还原调节和应激反应途径中的重要性。
种属

Human
表达系统

E. coli
标签

N-His
蛋白编号

P32119 (2A-198N)
基因 ID
蛋白结构
N-term
His
PRDX2 (2A-198N)
Accession # P32119
C-term
同用名
Epididymis secretory sperm binding protein Li 2a; HEL S 2a; MGC4104; Natural killer cell enhancing factor B; Natural killer cell-enhancing factor B; Natural Killer Enhancing Factor B; NKEF B; NKEF-B; NKEFB; Peroxiredoxin 2; Peroxiredoxin-2; PRDX 2; PRDX2; PrP; PRX2; PRXII; PTX1; TDPX1; Thiol Specific Antioxidant 1 ; Thiol specific antioxidant protein; Thiol-specific antioxidant protein; Thioredoxin Dependent Peroxide Reductase 1; Thioredoxin peroxidase 1; Thioredoxin-dependent peroxide reductase 1; Torin; TPX1; TSA
氨基酸序列

ASGNARIGKPAPDFKATAVVDGAFKEVKLSDYKGKYVVLFFYPLDFTFVCPTEIIAFSNRAEDFRKLGCEVLGVSVDSQFTHLAWINTPRKEGGLGPLNIPLLADVTRRLSEDYGVLKTDEGIAYRGLFIIDGKGVLRQITVNDLPVGRSVDEALRLVQAFQYTDEHGEVCPAGWKPGSDTIKPNVDDSKEYFSKHN

分子量

Approximately 25.8 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Peroxiredoxin-2/PRDX2 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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