PFKFB3 蛋白, Human (His)

The PFKFB3 protein is a key player in cellular energy metabolism as it exerts dual functions by catalyzing the synthesis and degradation of fructose 2,6-bisphosphate (F-2,6-BP). F-2,6-BP is an important allosteric regulator of glycolysis, controlling glucose utilization for energy production. PFKFB3 Protein, Human (His) is the recombinant human-derived PFKFB3 protein, expressed by E. coli , with N-6*His labeled tag. The total length of PFKFB3 Protein, Human (His) is 520 a.a., with molecular weight of 57-62 kDa.

PFKFB3 蛋白是细胞能量代谢的关键参与者，因为它通过催化果糖 2,6-二磷酸 (F-2,6-BP) 的合成和降解发挥双重功能。F-2,6-BP 是糖酵解的重要变构调节剂，可控制能量生产中葡萄糖的利用率。PFKFB3 作为生成 F-2,6-BP 的激酶，促进糖酵解限速酶磷酸果糖激酶-1 (PFK-1) 的激活。这导致糖酵解通量增加和能量产生增加。相反，PFKFB3也可以作为磷酸酶降解F-2,6-BP，对PFK-1产生抑制作用，从而减缓糖酵解。PFKFB3 活性的严格调节对于维持细胞能量稳态至关重要，并对各种生理和病理过程（包括癌症和代谢紊乱）产生影响。了解控制 PFKFB3 的复杂调节机制可以为针对糖酵解代谢的治疗策略提供见解。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

Q16875 (M1-H520)

5209  [NCBI]

MPLELTQSRVQKIWVPVDHRPSLPRSCGPKLTNSPTVIVMVGLPARGKTYISKKLTRYLNWIGVPTKVFNVGEYRREAVKQYSSYNFFRPDNEEAMKVRKQCALAALRDVKSYLAKEGGQIAVFDATNTTRERRHMILHFAKENDFKAFFIESVCDDPTVVASNIMEVKISSPDYKDCNSAEAMDDFMKRISCYEASYQPLDPDKCDRDLSLIKVIDVGRRFLVNRVQDHIQSRIVYYLMNIHVQPRTIYLCRHGENEHNLQGRIGGDSGLSSRGKKFASALSKFVEEQNLKDLRVWTSQLKSTIQTAEALRLPYEQWKALNEIDAGVCEELTYEEIRDTYPEEYALREQDKYYYRYPTGESYQDLVQRLEPVIMELERQENVLVICHQAVLRCLLAYFLDKSAEEMPYLKCPLHTVLKLTPVAYGCRVESIYLNVESVCTHRERSEDAKKGPNPLMRRNSVTPLASPEPTKKPRINSFEEHVASTSAALPSCLPPEVPTQLPGQNMKGSRSSADSSRKH

57-62 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM PB, 50 mM KCl, 5 mM DTT, 0.1 mM EDTA, 50% glycerol, 0.1% TritonX-100, pH 6.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.