PGK1 蛋白, Mouse (sf9, His)

The PGK1 protein catalyzes the conversion of 1,3-bisphosphoglycerate into 3-phosphoglycerate and plays a central role in ATP production.It also acts as a polymerase alpha cofactor protein and may be involved in primer recognition.PGK1 Protein, Mouse (sf9, His) is the recombinant mouse-derived PGK1 protein, expressed by Sf9 insect cells , with C-His labeled tag.

PGK1 蛋白是细胞能量动力学的关键贡献者，通过催化 1,3-二磷酸甘油酸可逆转化为 3-磷酸甘油酸，协调糖酵解途径的基本步骤，从而在 ATP 产生中发挥核心作用。除了其已确定的糖酵解功能之外，PGK1 还表现出多功能性，因为它似乎充当聚合酶 α 辅因子蛋白，可能参与引物识别过程。这种双重功能凸显了 PGK1 的重要性超出了其在糖酵解中的典型作用。此外，有一个有趣的建议表明 PGK1 可能与精子活力有关，暗示其可能参与生殖过程。从本质上讲，PGK1 作为一种多方面的蛋白质出现，影响能量代谢和细胞功能，从而导致细胞生理学的复杂性。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Mouse

Sf9 insect cells

C-His

P09411 (M1-V417)

18655  [NCBI]

Approximately 46 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.