PHGDH 蛋白, Human (C-His)

PHGDH protein plays a key role in cellular metabolism by catalyzing the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, marking the first step in the phosphorylated L-serine biosynthetic pathway.PHGDH Protein, Human (C-His) is the recombinant human-derived PHGDH protein, expressed by E.coli , with C-6*His labeled tag.

PHGDH（磷酸甘油酸脱氢酶）是一种在细胞代谢中发挥关键作用的酶，通过催化 3-磷酸-D-甘油酸可逆氧化为 3-膦酰氧基丙酮酸，标志着磷酸化 L-丝氨酸生物合成途径的第一步。该途径对于 L-丝氨酸的产生至关重要，L-丝氨酸是各种细胞成分（包括核苷酸和蛋白质）的前体。除了在丝氨酸生物合成中的作用外，PHGDH 还表现出多种酶活性，包括 2-羟基戊二酸可逆氧化为 2-酮戊二酸以及 (S)-苹果酸可逆氧化为草酰乙酸。这些额外的活性表明 PHGDH 参与调节代谢通量和维持细胞氧化还原平衡。了解 PHGDH 的功能可以深入了解复杂的代谢途径网络，并强调其在支持基本细胞过程中的重要性。参考资料：所提供的信息基于截至 2022 年 1 月的一般知识，未引用具体参考资料。

Measured by the ability to catalyze the oxidation of 3-phospho-D-glycerate.which can be measured by absorbance at 340 nm. The specific activity is 3159.161 pmol/min/µg, as measured under the described conditions.

Human

E. coli

C-6*His

O43175 (M1-F533)

26227  [NCBI]

MAFANLRKVLISDSLDPCCRKILQDGGLQVVEKQNLSKEELIAELQDCEGLIVRSATKVTADVINAAEKLQVVGRAGTGVDNVDLEAATRKGILVMNTPNGNSLSAAELTCGMIMCLARQIPQATASMKDGKWERKKFMGTELNGKTLGILGLGRIGREVATRMQSFGMKTIGYDPIISPEVSASFGVQQLPLEEIWPLCDFITVHTPLLPSTTGLLNDNTFAQCKKGVRVVNCARGGIVDEGALLRALQSGQCAGAALDVFTEEPPRDRALVDHENVISCPHLGASTKEAQSRCGEEIAVQFVDMVKGKSLTGVVNAQALTSAFSPHTKPWIGLAEALGTLMRAWAGSPKGTIQVITQGTSLKNAGNCLSPAVIVGLLKEASKQADVNLVNAKLLVKEAGLNVTTSHSPAAPGEQGFGECLLAVALAGAPYQAVGLVQGTTPVLQGLNGAVFRPEVPLRRDLPLLLFRTQTSDPAMLPTMIGLLAEAGVRLLSYQTSLVSDGETWHVMGISSLLPSLEAWKQHVTEAFQFHF

Approximately 57 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, 200 mM arginine, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.