2. 重组蛋白
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. Phytanoyl-CoA dioxygenase 蛋白, Actinophytocola oryzae

Phytanoyl-CoA dioxygenase 蛋白, Actinophytocola oryzae

目录号: HY-P702105
产品使用指南

植酰辅酶A双加氧酶 (PHYH) 是一种参与植烷酸（一种支链脂肪酸）α-氧化的酶。PHYH 催化植酰辅酶 A 转化为 2-羟基植酰辅酶 A，启动降解途径。Phytanoyl-CoA dioxygenase 蛋白, Actinophytocola oryzae 是重组的 植酰辅酶A双加氧酶蛋白，由 E. coli 表达，不带标签。Phytanoyl-CoA dioxygenase 蛋白, Actinophytocola oryzae 全长 266 个氨基酸。

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描述

Phytyl-CoA dioxygenase (PHYH) is an enzyme involved in the alpha-oxidation of phytanic acid, a branched-chain fatty acid. PHYH catalyzes the conversion of phytyl-CoA to 2-hydroxyphytyl-CoA, initiating the degradation pathway. Phytanoyl-CoA dioxygenase Protein, Actinophytocola oryzae is the recombinant Phytanoyl-CoA dioxygenase protein, expressed by E. coli , with tag free. The total length of Phytanoyl-CoA dioxygenase Protein, Actinophytocola oryzae is 266 a.a., .
研究背景

植酰辅酶A双加氧酶 (PHYH) 是一种对于植烷酸（一种支链脂肪酸）的 α 氧化至关重要的酶。 PHYH 催化植酰辅酶 A 转化为 2-羟基植酰辅酶 A，启动降解途径。 这一过程对于防止植烷酸的积累至关重要，植烷酸与雷夫苏姆病有关。 PHYH 的作用强调了其在维持脂质稳态和预防代谢紊乱方面的重要性。
种属

Others
表达系统

E. coli
标签

Tag Free
蛋白编号

A0A4R7W2E1 (M1-F266)
基因 ID

/
蛋白结构
N-term
PhyH (M1-F266)
Accession # A0A4R7W2E1
C-term
同用名
Ectoine hydroxylase
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Phytanoyl-CoA dioxygenase Protein, Actinophytocola oryzae 相关分类

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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