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  3. PIGR 蛋白, Human (HEK293, His)

PIGR 蛋白, Human (HEK293, His)

目录号: HY-P71065
COA 产品使用指南

PIGR 蛋白在介导聚合 IgAIgM 跨粘膜上皮细胞的选择性转胞吞作用中发挥关键作用,这对于粘膜免疫至关重要。PIGR 在基底外侧表面结合这些免疫球蛋白,形成在细胞内运输并在顶部分泌的复合物。PIGR 蛋白, Human (HEK293, His) 是重组的 PIGR 蛋白,由 HEK293 表达,带有 C-6*His 标签。

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规格 价格 是否有货 数量
10 μg ¥1600 In-stock
50 μg ¥4500 In-stock
100 μg   询价  

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描述

PIGR proteins play a critical role in mediating the selective transcytosis of polymerized IgA and IgM across mucosal epithelial cells, which is critical for mucosal immunity. PIGR binds these immunoglobulins at the basolateral surface, forming a complex that is transported intracellularly and secreted apically. PIGR Protein, Human (HEK293, His) is the recombinant human-derived PIGR protein, expressed by HEK293 , with C-6*His labeled tag.

研究背景

PIGR 蛋白在介导聚合 IgAIgM 跨粘膜上皮细胞的选择性转胞吞作用中发挥着至关重要的作用,协调粘膜免疫所必需的过程。该蛋白与上皮细胞基底外侧表面的聚合 IgAIgM 结合,形成复合物,随后转运穿过细胞并在顶端表面分泌。在此转运过程中,发生裂解事件,将细胞外成分(称为分泌成分)与跨膜片段分离。PIGR 通过其 N 连接聚糖确保分泌型 IgA (sIgA) 分子锚定在上皮表面的粘液上,这是中和细胞外病原体的关键机制。游离形式的 PIGR 还可以充当非特异性微生物清道夫,在防止病原体与上皮细胞相互作用方面发挥作用,并有助于更广泛地防御潜在感染。

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

P01833 (K19-R638, G365S)

基因 ID
蛋白结构
N-term
PIGR (K19-R638, G365S)
Accession # P01833
6*His
C-term
同用名
Polymeric Immunoglobulin Receptor; PIgR; Poly-Ig Receptor; Hepatocellular Carcinoma-Associated Protein TB6; PIGR
氨基酸序列

KSPIFGPEEVNSVEGNSVSITCYYPPTSVNRHTRKYWCRQGARGGCITLISSEGYVSSKYAGRANLTNFPENGTFVVNIAQLSQDDSGRYKCGLGINSRGLSFDVSLEVSQGPGLLNDTKVYTVDLGRTVTINCPFKTENAQKRKSLYKQIGLYPVLVIDSSGYVNPNYTGRIRLDIQGTGQLLFSVVINQLRLSDAGQYLCQAGDDSNSNKKNADLQVLKPEPELVYEDLRGSVTFHCALGPEVANVAKFLCRQSSGENCDVVVNTLGKRAPAFEGRILLNPQDKDGSFSVVITGLRKEDAGRYLCGAHSDGQLQEGSPIQAWQLFVNEESTIPRSPTVVKGVAGGSVAVLCPYNRKESKSIKYWCLWEGAQNGRCPLLVDSEGWVKAQYEGRLSLLEEPGNGTFTVILNQLTSRDAGFYWCLTNGDTLWRTTVEIKIIEGEPNLKVPGNVTAVLGETLKVPCHFPCKFSSYEKYWCKWNNTGCQALPSQDEGPSKAFVNCDENSRLVSLTLNLVTRADEGWYWCGVKQGHFYGETAAVYVAVEERKAAGSRDVSLAKADAAPDEKVLDSGFREIENKAIQDPRLFAEEKAVADTRDQADGSRASVDSGSSEEQGGSSR

分子量

Approximately 88.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料

PIGR Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PIGR Protein, Human (HEK293, His)
目录号:
HY-P71065
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