1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. PKM2 蛋白, Human (530a.a, His)

PKM2 蛋白, Human (530a.a, His)

目录号: HY-P70599
COA 产品使用指南

PKM2 蛋白, Human (530a.a, His) 是带有 His 标签的重组 PKM2 蛋白。PKM2 蛋白, 是糖酵解中的限速酶之一。

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规格 价格 是否有货 数量
10 μg ¥880 In-stock
50 μg ¥2450 询价
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

PKM2 Protein, Human (530a.a, His) is a recombinant PKM2 protein with a His-Flag. PKM2 Protein is one of the rate-limiting enzymes in glycolysis[3].

研究背景

PKM2在增殖细胞中特异表达,如胚胎干细胞、胚胎癌细胞和各种癌细胞。
PKM2作为糖酵解酶,催化磷酸烯醇丙酮酸(PEP)的磷酰基转移到ADP,产生ATP。PKM2 是糖酵解中的限速酶之一,与线粒体融合的关键调节因子线粒体融合素 2 (MFN2) 相互作用,促进线粒体融合和 OXPHOS,并减弱糖酵解[1] [2][3]

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

P14618-1 (S2-P531)

基因 ID
蛋白结构
N-term
6*His
PKM2 (S2-P531)
Accession # P14618-1
C-term
同用名
Pyruvate kinase PKM; CTHBP; OIP-3; THBP1
氨基酸序列

SKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP

分子量

Approximately 63.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 50 mM NaCl, 0.05% Brij-35, 1 mM DTT, pH 7.5.

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料
参考文献

PKM2 Protein, Human (530a.a, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PKM2 Protein, Human (530a.a, His)
目录号:
HY-P70599
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