1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. PKM2 蛋白, Human

PKM2 蛋白, Human

目录号: HY-P702845
产品使用指南

PKM2 蛋白, Human 是无 标签的重组 PKM2 蛋白。PKM2 蛋白, 是糖酵解中的限速酶之一。

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规格 是否有货
50 μg   询价  
100 μg   询价  

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描述

PKM2 Protein, Human is a recombinant PKM2 protein with no tagged. PKM2 Protein is one of the rate-limiting enzymes in glycolysis[3].

研究背景

PKM2在增殖细胞中特异表达,如胚胎干细胞、胚胎癌细胞和各种癌细胞。
PKM2作为糖酵解酶,催化磷酸烯醇丙酮酸(PEP)的磷酰基转移到ADP,产生ATP。PKM2 是糖酵解中的限速酶之一,与线粒体融合的关键调节因子线粒体融合素 2 (MFN2) 相互作用,促进线粒体融合和 OXPHOS,并减弱糖酵解[1] [2][3]

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

P14618-1 (M1-P531)

基因 ID

5315

蛋白结构
N-term
PKM2 (M1-P531)
Accession # P14618-1
C-term
同用名
Pyruvate kinase PKM; CTHBP; OIP-3; THBP1
氨基酸序列

SKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP

分子量

57.9 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PKM2 Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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