PLA2G1B 蛋白, Human (HEK293, His)

The PLA2G1B protein is a secreted calcium-dependent phospholipase A2 that targets dietary phospholipids in the intestine. It hydrolyzes the fatty acyl group at the sn-2 position, preferentially hydrolyzing phosphatidylethanolamine and phosphatidylglycerol. PLA2G1B Protein, Human (HEK293, His) is the recombinant human-derived PLA2G1B protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PLA2G1B Protein, Human (HEK293, His) is 126 a.a., with molecular weight of 17-20 kDa.

PLA2G1B 蛋白是一种分泌型钙依赖性磷脂酶 A2，主要针对肠道中的膳食磷脂。它水解磷脂 sn-2 位上脂肪酰基的酯键，优先水解磷脂酰乙醇胺和磷脂酰甘油而不是磷脂酰胆碱。这种蛋白质可能在 N-酰基乙醇胺的生物合成中发挥作用，调节肠道能量代谢和炎症。它还将 N-酰基磷脂酰乙醇胺水解为 N-酰基溶血磷脂酰乙醇胺，后者被溶血磷脂酶 D 进一步裂解，释放 N-酰基乙醇胺。它可能以自分泌和旁分泌的方式起作用，当与 PLA2R1 受体结合时，可以调节足细胞的存活和肾小球稳态。此外，PLA2G1B 具有抗蠕虫活性，该活性受肠道微生物群调节。当蠕虫感染肠上皮时，它直接影响蠕虫幼虫膜中磷脂酰乙醇胺的含量，可能控制膜融合和细胞分裂等过程，同时帮助免疫识别。这最终会降低幼虫的完整性和传染性。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

P04054 (A23-S148)

5319  [NCBI]

AVWQFRKMIKCVIPGSDPFLEYNNYGCYCGLGGSGTPVDELDKCCQTHDNCYDQAKKLDSCKFLLDNPYTHTYSYSCSGSAITCSSKNKECEAFICNCDRNAAICFSKAPYNKAHKNLDTKKYCQS

17-20 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 10% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.