PLA2G7 蛋白, Human (HEK293, His)

PLA2G7 Protein is a lipoprotein-associated calcium-independent phospholipase A2 involved in phospholipid catabolism during inflammatory and oxidative stress responses. It is also a secreted enzyme that catalyzes the degradation of platelet-activating factor to biologically inactive products. PLA2G7 can hydrolyze phospholipids with long fatty acyl chains, only if they carry oxidized functional groups. PLA2G7 Protein, Human (HEK293, His) is the recombinant human-derived PLA2G7 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PLA2G7 Protein, Human (HEK293, His) is 420 a.a., with molecular weight of ~57 kDa.

血小板激活因子乙酰水解酶 (PLA2G7) 是一种脂蛋白相关的钙非依赖性磷脂酶 A2，参与炎症和氧化应激反应期间的磷脂分解代谢。在脂质-水界面，PLA2G7 水解磷脂 sn-2 位上脂肪酰基的酯键，特异性靶向 sn-2 位上具有短链脂肪酰基的磷脂。它还可以水解并灭活血小板激活因子 (PAF, 1-O-烷基-2-乙酰基-sn-甘油-3-磷酸胆碱)，这是一种有效的促炎信号脂质，通过 PTAFR 作用于各种先天免疫细胞。此外，PLA2G7 可以水解在 omega 位置带有醛基的氧化截短的磷脂，防止其在低密度脂蛋白 (LDL) 颗粒中积聚以及不受控制的促炎作用。作为高密度脂蛋白 (HDL) 颗粒的一部分，PLA2G7 可以水解在 sn-2 位点具有长链脂肪酰基氢过氧化物的磷脂，并防止这些氧脂质在血管壁中潜在的积累。PLA2G7 催化 F2-异前列腺素从膜磷脂中释放，F2-异前列腺素是细胞氧化损伤的脂质生物标志物^{[1][2][3][4][5]}。

Measured by its ability to cleave a colorimetric peptide substrate of 200μM 2-Thio-PAF, in the presence of 5,5’Dithio-bis(2-nitrobenzoic acid) (DTNB) that incubate at room temperature for 5 minutes. The specific activity is 27117.898 pmol/min/ug.

Human

HEK293

C-6*His

AAH38452.1 (F22-N441)

7941  [NCBI]

FDWQYINPVAHMKSSAWVNKIQVLMAAASFGQTKIPRGNGPYSVGCTDLMFDHTNKGTFLRLYYPSQDNDRLDTLWIPNKEYFWGLSKFLGTHWLMGNILRLLFGSMTTPANWNSPLRPGEKYPLVVFSHGLGAFRTLYSAIGIDLASHGFIVAAVEHRDRSASATYYFKDQSAAEIGDKSWLYLRTLKQEEETHIRNEQVRQRAKECSQALSLILDIDHGKPVKNALDLKFDMEQLKDSIDREKIAVIGHSFGGATVIQTLSEDQRFRCGIALDAWMFPLGDEVYSRIPQPLFFINSEYFQYPANIIKMKKCYSPDKERKMITIRGSVHQNFADFTFATGKIIGHMLKLKGDIDSNAAIDLSNKASLAFLQKHLGLHKDFDQWDCLIEGDDENLIPGTNINTTNQHIMLQNSSGIEKYN

Approximately 57 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 50 mM NaAc, 150 mM NaCl, 10% Glycerol, pH 5.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.