PLY 蛋白, Streptococcus pneumoniae serotype 4 (Baculovirus, His-Myc)

The PLY protein, a cholesterol-dependent toxin, induces cytolysis by forming pores in host membranes with a significant conformational change and oligomeric pore complex formation. Cholesterol is crucial for binding, insertion, and pore formation. PLY Protein, Streptococcus pneumoniae serotype 4 (Baculovirus, His-Myc) is the recombinant PLY protein, expressed by Sf9 insect cells, with N-10*His, C-Myc labeled tag. The total length of PLY Protein, Streptococcus pneumoniae serotype 4 (Baculovirus, His-Myc) is 470 a.a., with molecular weight of ~56.8 kDa.

PLY 蛋白作为一种胆固醇依赖性毒素，通过在含有胆固醇的宿主膜上形成孔来诱导细胞溶解。与靶膜结合后，蛋白质经历显着的构象变化，促进其插入宿主膜并随后形成寡聚孔复合物。胆固醇的存在对于该过程的各个阶段都至关重要，包括与宿主膜的结合、膜插入和孔形成，其中胆固醇结合由 C 末端的 Thr-Leu 对介导。值得注意的是，PLY 蛋白具有可逆性，可以通过氧化失活。所得同源寡聚孔复合物包含 35 至 50 个亚基，在插入宿主膜后形成，有助于蛋白质的溶细胞活性。

Others

Sf9 insect cells

N-10*His;C-Myc

P0C2J9 (A2-D471)

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ANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFVVIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSIDLPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQLKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTVTVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQTEWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTSFLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVLTPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND

Approximately 56.8 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.