HMBS/Porphobilinogen deaminase 蛋白, Human (HEK293, His)

HMBS (porphobilinogen deaminase) is key to heme biosynthesis, catalyzing the sequential polymerization of four porphobilinogen molecules to form hydroxymethylbiphenyl. The process begins with dipyrromethane cofactor assembly derived from porphobilinogen or preuroporphyrinogen and promoted by apoenzymes. HMBS/Porphobilinogen deaminase Protein, Human (HEK293, His) is the recombinant human-derived HMBS/Porphobilinogen deaminase protein, expressed by HEK293 , with C-6*His labeled tag.

HMBS，也称为胆色素原脱氨酶，通过催化四分子胆色素原的顺序聚合，导致形成羟甲基联烷，也称为前尿卟啉原，在血红素生物合成途径中发挥着至关重要的作用。催化过程始于二吡咯甲烷辅因子的组装，二吡咯甲烷辅因子源自两个胆色素原分子或前尿卟啉原，由脱辅基酶组装而成。这种共价连接的辅因子充当引物，促进四吡咯产物的组装。在催化的最后一步，产物前尿卟啉原被释放，留下完整的辅因子与全脱氨酶结合。这种酶活性对于血红素的合成至关重要，血红素是具有多种生物功能的重要成分。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

P08397-1 (S2-H361)

3145  [NCBI]

SGNGNAAATAEENSPKMRVIRVGTRKSQLARIQTDSVVATLKASYPGLQFEIIAMSTTGDKILDTALSKIGEKSLFTKELEHALEKNEVDLVVHSLKDLPTVLPPGFTIGAICKRENPHDAVVFHPKFVGKTLETLPEKSVVGTSSLRRAAQLQRKFPHLEFRSIRGNLNTRLRKLDEQQEFSAIILATAGLQRMGWHNRVGQILHPEECMYAVGQGALGVEVRAKDQDILDLVGVLHDPETLLRCIAERAFLRHLEGGCSVPVAVHTAMKDGQLYLTGGVWSLDGSDSIQETMQATIHVPAQHEDGPEDDPQLVGITARNIPRGPQLAAQNLGISLANLLLSKGAKNILDVARQLNDAH

Approximately 47.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 5% Trehalose, 5% mannitol, 50% Glycerol, 0.1% Tween80, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.