PPM1H 蛋白, Human (HEK293, His)

PPM1H, the enzyme subject, dephosphorylates CDKN1B at 'Thr-187,' critically regulating cellular processes. By preventing proteasomal degradation, PPM1H stabilizes CDKN1B, emphasizing its role in protein stabilization. This dephosphorylation event, orchestrated by PPM1H, intricately controls CDKN1B levels, showcasing its involvement in modulating cell cycle progression and regulating key cellular functions. PPM1H, Human (HEK293, His) is the recombinant human-derived PPM1H, expressed by HEK293 , with C-His labeled tag. The total length of PPM1H, Human (HEK293, His) is 514 a.a., with molecular weight of ~75 kDa.

受试者 PPM1H 充当一种酶，使 CDKN1B 在“Thr-187”处去磷酸化，从而在细胞过程的调节中发挥关键作用。通过消除该特定位点的磷酸化信号，PPM1H 可防止 CDKN1B 的蛋白酶体降解，从而强调其稳定蛋白质的作用。这种由 PPM1H 精心策划的去磷酸化事件有助于对 CDKN1B 水平的复杂控制，强调了该酶参与细胞周期进程的调节和关键细胞功能的调节。

Human

HEK293

C-His

Q9ULR3 (M1-S514)

57460

MLTRVKSAVANFMGGIMAGSSGSEHGGGSCGGSDLPLRFPYGRPEFLGLSQDEVECSADHIARPILILKETRRLPWATGYAEVINAGKSTHNEDQASCEVLTVKKKAGAVTSTPNRNSSKRRSSLPNGEGLQLKENSESEGVSCHYWSLFDGHAGSGAAVVASRLLQHHITEQLQDIVDILKNSAVLPPTCLGEEPENTPANSRTLTRAASLRGGVGAPGSPSTPPTRFFTEKKIPHECLVIGALESAFKEMDLQIERERSSYNISGGCTALIVICLLGKLYVANAGDSRAIIIRNGEIIPMSSEFTPETERQRLQYLAFMQPHLLGNEFTHLEFPRRVQRKELGKKMLYRDFNMTGWAYKTIEDEDLKFPLIYGEGKKARVMATIGVTRGLGDHDLKVHDSNIYIKPFLSSAPEVRIYDLSKYDHGSDDVLILATDGLWDVLSNEEVAEAITQFLPNCDPDDPHRYTLAAQDLVMRARGVLKDRGWRISNDRLGSGDDISVYVIPLIHGNKLS

Approximately 75 kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of 25 mM Tris-HCl, 150 mM NaCl, pH 7.4, 10% Glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.