1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. PPT1 蛋白, Mouse (HEK293, His)

PPT1 蛋白, Mouse (HEK293, His)

目录号: HY-P700622
COA 产品使用指南

PPT1 蛋白是一种溶酶体降解酶,可从蛋白质或肽中修饰的半胱氨酸残基中去除硫酯连接的脂肪酰基,例如棕榈酸酯。它更喜欢 14 至 18 个碳的酰基链长度。PPT1 蛋白, Mouse (HEK293, His) 是重组的 PPT1 蛋白,由 HEK293 表达,带有 C-10*His 标签。

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规格 价格 是否有货 数量
20 μg ¥3100 In-stock
50 μg ¥6800 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

PPT1 Protein, an enzyme in lysosomal degradation, crucially removes thioester-linked fatty acyl groups, like palmitate, from modified cysteine residues in proteins or peptides.It prefers acyl chain lengths from 14 to 18 carbons.PPT1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived PPT1 protein, expressed by HEK293 , with C-10*His labeled tag.

研究背景

PPT1 蛋白是一种参与溶酶体降解的酶,在从蛋白质或肽中修饰的半胱氨酸残基中去除硫酯连接的脂肪酰基(例如棕榈酸酯)方面发挥着至关重要的作用。它表现出对 14 至 18 个碳的酰基链长度的偏好。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Mouse

表达系统

HEK293

标签

C-10*His

蛋白编号

O88531 (D28-K306)

基因 ID
蛋白结构
N-term
PPT1 (D28-K306)
Accession # O88531
10*His
C-term
同用名
Palmitoyl-protein thioesterase 1; PPT-1; Palmitoyl-protein hydrolase 1; PPT1
氨基酸序列

DPPSPPPLVIWHGMGDSCCNPMSMGVIKKMVEKEIPGIYVLSLEIGKNMMEDVENSFFLNVNVQVNMVCQILEKDPKLQQGYNAIGFSQGGQFLRAVAQRCPTPPMMTLISVGGQHQGVFGLPRCPGESSHICDFIRKSLNAGAYSKLVQERLVQAQYWHDPIKESVYRNYSIFLADINQERCVNESYKKNLMALKKFVMVKFFNDSIVDPVDSEWFGFYRSGQAKETIPLQESTLYTEDRLGLKKMDKAGKLVFLAKEGDHLQISKEWFTAHIIPFLK

分子量

33 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

PPT1 Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PPT1 Protein, Mouse (HEK293, His)
目录号:
HY-P700622
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