PRC1 蛋白, Human (sf9, His)

PRC1 is a key regulator of cytokinesis, coordinating midzone formation and successful cell division by cross-linking microtubules and recruiting essential proteins such as KIF14, PLK1 and ECT2. Its effects extend to tumorigenesis, promoting bladder cancer cell proliferation and inhibiting apoptosis. PRC1 Protein, Human (sf9, His) is the recombinant human-derived PRC1 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of PRC1 Protein, Human (sf9, His) is 620 a.a., with molecular weight of ~75 kDa.

PRC1 是胞质分裂的关键调节因子，通过以平均距离 35 nM 交联反平行微管，在协调中区的时空形成并确保胞质分裂成功方面发挥着至关重要的作用。它的不可或缺性还包括将 KIF14、PLK1 和 ECT2 等关键蛋白招募到中央纺锤体和中体，这是正确细胞分裂的重要步骤。除了在细胞过程中的基本作用外，PRC1 还表现出致癌特性，促进增殖、抑制细胞凋亡，并促进膀胱癌细胞的致癌进展。作为同型二聚体，PRC1 与多种蛋白质相互作用，包括RACGAP1、CENPE、KIF4A、KIF23 和 PLK1，形成复杂的分子相互作用网络，对其有丝分裂期间的多方面功能至关重要。PRC1 与这些蛋白质的复杂相互作用强调了它在控制细胞分裂的复杂机制中的重要性，并强调了它作为癌症治疗干预靶点的潜力。

Human

Sf9 insect cells

N-His

O43663 (M1-S620)

9055  [NCBI]

Approximately 75 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 20% Glycerol, 3 mM DTT. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.