PRKAR1A 蛋白, Human (HEK293, His)

PRKAR1A protein is a regulatory subunit of cAMP-dependent protein kinase and is critical for cAMP signaling. Upon activation by cAMP, it releases two catalytic monomers and a regulatory dimer from the inactive holoenzyme. PRKAR1A Protein, Human (HEK293, His) is the recombinant human-derived PRKAR1A protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PRKAR1A Protein, Human (HEK293, His) is 380 a.a., with molecular weight of ~50.0 kDa.

PRKAR1A 蛋白是 cAMP 依赖性蛋白激酶的调节亚基，在细胞内 cAMP 信号传导中发挥着至关重要的作用。无活性的全酶由两条调节链和两条催化链组成，它们在 cAMP 激活后释放，导致形成两个活性催化单体和调节二聚体。PRKAR1A 蛋白与 PRKACA 和 PRKACB 相互作用，并且这种相互作用已被证明会影响各种细胞过程。此外，PRKAR1A 蛋白与 RFC2 结合，可能有助于细胞存活。它还与 AKAP4、RARA（在 cAMP 或 FSH 存在下，调节 RARA 转录活性）、磷酸化 PJA2、CBFA2T3（通过相似性）、PRKX（调节这种 cAMP 依赖性蛋白激酶）、C2orf88/smAKAP（可能靶向 PRKAR1A质膜）和 AICDA，进一步强调了它参与多种分子相互作用和途径。

Human

HEK293

C-6*His

P10644 (E2-V381)

5573  [NCBI]

ESGSTAASEEARSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKEEAKQIQNLQKAGTRTDSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFSVSFIAGETVIQQGDEGDNFYVIDQGETDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPGDEFFIILEGSAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPRAATVVARGPLKCVKLDRPRFERVLGPCSDILKRNIQQYNSFVSLSV

Approximately 50.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.