Profilin-2 蛋白, Human (His)

Profilin-2 protein is a key player in protein folding. It specifically binds to cytosolic chaperone protein (c-CPN) and promotes the transfer of target proteins. It also interacts with nascent polypeptide chains, helping unnatural proteins fold correctly in various competing pathways. Profilin-2 Protein, Human (His) is the recombinant human-derived Profilin-2 protein, expressed by E. coli , with N-6*His labeled tag.

PFDN2 是蛋白质折叠中的关键参与者，对胞质伴侣蛋白 (c-CPN) 表现出特异性结合亲和力，促进目标蛋白质向该复合物的转移。此外，PFDN2 与新生多肽链相互作用，促进其在存在各种非天然蛋白质竞争途径的环境中正确折叠。PFDN2的异六聚体结构包含两个PFD-α型和四个PFD-β型亚基。此外，PFDN2 是 PAQosome 复合物的一个组成部分，与其他成员（如 RUVBL1、RUVBL2、RPAP3、PIH1D1、PFDN6、PDRG1、UXT、URI1、ASDURF、POLR2E 和 DNAAF10/WDR92）在多种蛋白质复合物的生物合成中合作。值得注意的是，PFDN2 和 URI1 之间的相互作用是磷酸化依赖性的，并表现出生长依赖性模式，突出了细胞过程中涉及的复杂调节机制。

Human

E. coli

N-6*His

AAH18049.1 (M1-F140)

5217  [NCBI]

MAGWQSYVDNLMCDGCCQEAAIVGYCDAKYVWAATAGGVFQSITPIEIDMIVGKDREGFFTNGLALGAKKCSVIRDSLYVDGDCTMDIRTKSQGGEPTYNVAVGRAGRVLVFVMGKEGVHGGGLNKKAYSMAKYLRDSGF

Approximately 16 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, 300 mM NaCl, 500 mM arginine, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.