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  3. Profilin-2 蛋白, Human (His)

Profilin-2 蛋白, Human (His)

目录号: HY-P73698
COA 产品使用指南

Profilin-2 蛋白是蛋白质折叠的关键参与者。它特异性结合胞质伴侣蛋白(c-CPN)并促进靶蛋白的转移。它还与新生的多肽链相互作用,帮助非天然蛋白质在各种竞争途径中正确折叠。Profilin-2 蛋白, Human (His) 是重组的 Profilin-2 蛋白,由 E. coli 表达,带有 N-6*His 标签。

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规格 价格 是否有货 数量
Free Sample   Apply now  
5 μg ¥300 In-stock
10 μg ¥480 In-stock
50 μg ¥1060 In-stock
100 μg ¥1700 In-stock
500 μg ¥4400 In-stock
1 mg ¥7000 In-stock
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描述

Profilin-2 protein is a key player in protein folding. It specifically binds to cytosolic chaperone protein (c-CPN) and promotes the transfer of target proteins. It also interacts with nascent polypeptide chains, helping unnatural proteins fold correctly in various competing pathways. Profilin-2 Protein, Human (His) is the recombinant human-derived Profilin-2 protein, expressed by E. coli , with N-6*His labeled tag.

研究背景

PFDN2 是蛋白质折叠中的关键参与者,对胞质伴侣蛋白 (c-CPN) 表现出特异性结合亲和力,促进目标蛋白质向该复合物的转移。此外,PFDN2 与新生多肽链相互作用,促进其在存在各种非天然蛋白质竞争途径的环境中正确折叠。PFDN2的异六聚体结构包含两个PFD-α型和四个PFD-β型亚基。此外,PFDN2 是 PAQosome 复合物的一个组成部分,与其他成员(如 RUVBL1、RUVBL2、RPAP3、PIH1D1、PFDN6、PDRG1、UXT、URI1、ASDURF、POLR2E 和 DNAAF10/WDR92)在多种蛋白质复合物的生物合成中合作。值得注意的是,PFDN2 和 URI1 之间的相互作用是磷酸化依赖性的,并表现出生长依赖性模式,突出了细胞过程中涉及的复杂调节机制。

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

AAH18049.1 (M1-F140)

基因 ID
蛋白结构
N-term
6*His
Profilin-2 (M1-F140)
Accession # AAH18049.1
C-term
同用名
Profilin-II; PFN2; Profilin-2; PFL
氨基酸序列

MAGWQSYVDNLMCDGCCQEAAIVGYCDAKYVWAATAGGVFQSITPIEIDMIVGKDREGFFTNGLALGAKKCSVIRDSLYVDGDCTMDIRTKSQGGEPTYNVAVGRAGRVLVFVMGKEGVHGGGLNKKAYSMAKYLRDSGF

分子量

Approximately 16 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, 300 mM NaCl, 500 mM arginine, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Profilin-2 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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