Prolactin 蛋白, Sheep (HEK293, His)

Prolactin Protein, Sheep, (HEK293, His) is a recombinant protein produced by HEK293 cells. Prolactin (PRL) is a 199-amino acid polypeptide hormone mainly synthesized by lactotrope cells in the anterior pituitary. In rodents, PRL has been detected in the preoptic area, hypothalamus, amygdala, and brainstem^[1].

PRL 作用由 PRL 受体介导，PRL 受体属于细胞因子 1 类受体超家族，以短亚型和长亚型存在。PRL 受体的长形式和短形式均在啮齿动物的视前区、下丘脑和杏仁核以及其他大脑区域中表达。PRL 对成年动物的多种生物学效应已被描述，其中许多与繁殖有关。PRL 会增加基底下丘脑中多巴胺的合成和周转，从而使其能够反馈抑制 PRL 释放^[1]。

Measured in a cell proliferation assay using Nb2-11 rat lymphoma cells. The ED₅₀ for this effect is 0.2578-0.09207 ng/mL.

Sheep

HEK293

C-6*His

P01240 (T31-C229)

443317  [NCBI]

TPVCPNGPGNCQVSLRDLFDRAVMVSHYIHNLSSEMFNEFDKRYAQGKGFITMALNSCHTSSLPTPEDKEQAQQTHHEVLMSLILGLLRSWNDPLYHLVTEVRGMKGVPDAILSRAIEIEEENKRLLEGMEMIFGQVIPGAKETEPYPVWSGLPSLQTKDEDARHSAFYNLLHCLRRDSSKIDTYLKLLNCRIIYNNNC

Approximately 25-30 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Roselli CE, et al. Prolactin expression in the sheep brain. Neuroendocrinology. 2008;87(4):206-215.  [Content Brief]