Prostasin/PRSS8 蛋白, Human (HEK293, His)

Prostasin/PRSS8, with trypsin-like cleavage specificity, crucially activates the epithelial sodium channel (ENaC) by cleaving its gamma subunits (SCNN1G). This protein forms a heterodimeric complex, consisting of light and heavy chains connected by a disulfide bond. Prostasin/PRSS8 Protein, Human (HEK293, His) is the recombinant human-derived Prostasin/PRSS8 protein, expressed by HEK293 , with C-His labeled tag. The total length of Prostasin/PRSS8 Protein, Human (HEK293, His) is 293 a.a., with molecular weight of ~40 kDa.

Prostasin/PRSS8 蛋白表现出类似胰蛋白酶的切割特异性，对多碱性底物具有特殊的亲和力。它通过激活伽玛亚基 (SCNN1G) 的裂解，在刺激上皮钠通道 (ENaC) 的活性方面发挥着至关重要的作用。在结构上，它由两条链（一条轻链和一条重链）组成，这两条链通过二硫键连接在一起，形成异二聚体复合物。

Measured by its ability to cleave the fluorogenic peptide substrate Boc-QAR-AMC. The specific activity is 121.6525 pmol/min/µg, as measured under the described conditions.

Human

HEK293

C-His

Q16651 (A30-R322)

5652  [NCBI]

AEGAEAPCGVAPQARITGGSSAVAGQWPWQVSITYEGVHVCGGSLVSEQWVLSAAHCFPSEHHKEAYEVKLGAHQLDSYSEDAKVSTLKDIIPHPSYLQEGSQGDIALLQLSRPITFSRYIRPICLPAANASFPNGLHCTVTGWGHVAPSVSLLTPKPLQQLEVPLISRETCNCLYNIDAKPEEPHFVQEDMVCAGYVEGGKDACQGDSGGPLSCPVEGLWYLTGIVSWGDACGARNRPGVYTLASSYASWIQSKVTELQPRVVPQTQESQPDSNLCGSHLAFSSAPAQGLLR

Approximately 40 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.