Transcription termination factor Rho 蛋白, E.coli (His)

The transcription terminator Rho protein plays a key role in promoting transcription termination through a mechanism that binds to nascent RNA, activates Rho's RNA-dependent ATPase activity, and subsequently releases mRNA from the DNA template. As an RNA-dependent NTPase, Rho utilizes all four ribonucleoside triphosphates as substrates, highlighting its versatility in nucleotide interactions during termination. Transcription termination factor Rho Protein, E.coli (His) is the recombinant E. coli-derived Transcription termination factor Rho protein, expressed by E. coli , with N-6*His labeled tag. The total length of Transcription termination factor Rho Protein, E.coli (His) is 419 a.a., with molecular weight of ~51.0 kDa.

转录终止因子 Rho 蛋白对于基因表达的调节至关重要，它通过明确的机制促进转录终止。该过程涉及 Rho 与新生 RNA 的结合，激活 Rho 的 RNA 依赖性 ATP 酶活性，以及随后从 DNA 模板中释放 mRNA。作为一种 RNA 依赖性 NTP 酶，Rho 利用所有四种核糖核苷三磷酸作为底物，突出了其在终止过程中核苷酸相互作用的多功能性。值得注意的是，Rho 的 ATP 酶活性容易受到双环霉素和二氢双环霉素的抑制，为 Rho 介导的转录终止的药理学调节提供了潜在途径。

E.coli

E. coli

N-6*His

P0AG30 (M1-S419)

948297  [NCBI]

MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAKSGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIRPPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTARVLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTEMQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNTVVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEEFKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGEIDAMEFLINKLAMTKTNDDFFEMMKRS

Approximately 51.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μ m sterile filtered 20 mM Tris-HCl, 0.5 M NaCl, 6%Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.