PSAT1 蛋白, Human (His)

The PSAT1 protein plays a central role in cellular metabolism by catalyzing the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and 3-hydroxy-2-oxo-4-phosphonooxybutyrate to phosphohydroxythreonine. These enzyme activities are key steps in the biosynthetic pathway of the essential amino acids serine and threonine. PSAT1 Protein, Human (His) is the recombinant human-derived PSAT1 protein, expressed by E. coli , with C-6*His labeled tag. The total length of PSAT1 Protein, Human (His) is 370 a.a., with molecular weight of ~40 kDa.

PSAT1（磷酸丝氨酸转氨酶 1）蛋白是一种催化 3-磷酸羟基丙酮酸可逆转化为磷酸丝氨酸以及 3-羟基-2-氧代-4-膦酰氧基丁酸可逆转化为磷酸羟基苏氨酸的酶。这种酶活性是丝氨酸生物合成途径的关键步骤，有助于必需氨基酸的合成以及核苷酸和蛋白质生物合成等细胞过程。PSAT1 产生的磷酸丝氨酸和磷酸羟基苏氨酸是产生丝氨酸和甘氨酸的前体，这两者对于各种代谢途径和细胞功能都至关重要。PSAT1 在丝氨酸生物合成中的作用强调了其在维持细胞稳态中的重要性，并为涉及氨基酸代谢失调的疾病的治疗方法提供了潜在的见解。参考资料：所提供的信息基于截至 2022 年 1 月的一般知识，未引用具体参考资料。

Measured by its ability to produce 3-phosphooxypyruvate. The specific activity is >130 pmol/min/μg.

Human

E. coli

C-6*His

Q9Y617-1 (M1-L370)

29968  [NCBI]

MDAPRQVVNFGPGPAKLPHSVLLEIQKELLDYKGVGISVLEMSHRSSDFAKIINNTENLVRELLAVPDNYKVIFLQGGGCGQFSAVPLNLIGLKAGRCADYVVTGAWSAKAAEEAKKFGTINIVHPKLGSYTKIPDPSTWNLNPDASYVYYCANETVHGVEFDFIPDVKGAVLVCDMSSNFLSKPVDVSKFGVIFAGAQKNVGSAGVTVVIVRDDLLGFALRECPSVLEYKVQAGNSSLYNTPPCFSIYVMGLVLEWIKNNGGAAAMEKLSSIKSQTIYEIIDNSQGFYVCPVEPQNRSKMNIPFRIGNAKGDDALEKRFLDKALELNMLSLKGHRSVGGIRASLYNAVTIEDVQKLAAFMKKFLEMHQL

Approximately 40 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.