PSME2 蛋白, Human (His)

PSME2 Protein, vital for immunoproteasome assembly, is crucial for efficient antigen processing. In collaboration with PSME1, the PA28 activator complex modifies the proteasome's cleavage pattern, actively contributing to class I binding peptide generation. The hexameric ring structure of this heterodimeric complex underscores its significance in orchestrating processes essential for immune responses and antigen presentation. PSME2 Protein, Human (His) is the recombinant human-derived PSME2 protein, expressed by E. coli , with N-His labeled tag. The total length of PSME2 Protein, Human (His) is 239 a.a., with molecular weight of ~29 kDa.

PSME2 蛋白在免疫蛋白酶体组装中发挥着至关重要的作用，对于有效的抗原加工至关重要。PA28 激活剂复合物由 PSME1 和 PSME2 异二聚体组成，通过改变蛋白酶体的切割模式积极促进 I 类结合肽的生成。这种异二聚复合物形成六聚环结构，突出了其在协调对免疫反应和抗原呈递至关重要的过程中的重要性。

Immobilized Mouse PSME2 at 2 μg/mL (100 μL/well) can bind Anti- PSME2 Antibody, The ED₅₀ for this effect is 26.49 ng/mL.

Human

E. coli

N-His

Q9UL46 (M1-Y239)

5721  [NCBI]

MAKPCGVRLSGEARKQVEVFRQNLFQEAEEFLYRFLPQKIIYLNQLLQEDSLNVADLTSLRAPLDIPIPDPPPKDDEMETDKQEKKEVHKCGFLPGNEKVLSLLALVKPEVWTLKEKCILVITWIQHLIPKIEDGNDFGVAIQEKVLERVNAVKTKVEAFQTTISKYFSERGDAVAKASKETHVMDYRALVHERDEAAYGELRAMVLDLRAFYAELYHIISSNLEKIVNPKGEEKPSMY

Approximately 29 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 8.0, 10% Glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.