PSME3 蛋白, Human (N-His)

PSME3 is a subunit of the 11S REG-gamma proteasome modulator that forms a homoheptamer and is essential for activating the trypsin-like subunit of the proteasome and inhibiting the chymotrypsin-like and PGPH subunits. PSME3 promotes MDM2-p53 interaction, leading to p53 degradation and inhibiting apoptosis after DNA damage. PSME3 Protein, Human (N-His) is the recombinant human-derived PSME3 protein, expressed by E. coli , with N-His labeled tag.

PSME3 是 11S REG-gamma（也称为 PA28-gamma）蛋白酶体调节因子的亚基，形成与蛋白酶体结合的环形同七聚体。这种同七聚体在激活蛋白酶体的胰蛋白酶样催化亚基同时抑制胰凝乳蛋白酶样和谷氨酰后偏好 (PGPH) 亚基方面发挥着至关重要的作用。值得注意的是，PSME3 促进 MDM2 和 p53/TP53 之间的相互作用，导致 p53/TP53 泛素化和 MDM2 依赖性蛋白酶体降解。这一过程限制了 p53/TP53 的积累，从而抑制 DNA 损伤后的细胞凋亡。此外，PSME3 可能有助于细胞周期调节。PSME3 同七聚体的稳定性对于其特异性激活胰蛋白酶样亚基以及抑制蛋白酶体的胰凝乳蛋白酶样和 PGPH 亚基至关重要。PSME3 与多种蛋白质相互作用，包括 MAP3K3、CCAR2 和 PSME3IP1，后者直接促进 PSME3 与 20S 蛋白酶体的结合。PSME3IP1 抑制与 COIL 的相互作用。这种复杂的相互作用网络强调了 PSME3 在调节蛋白酶体活性和影响关键细胞过程中的多方面作用。

Measured by its ability to cleave a fluorogenic peptide substrate, suc-LLVY-AMC (HY-P1002). The specific activity is 897.05 pmol/min/μg.

Human

E. coli

N-6*His

P61289 (M1-Y254)

10197

MASLLKVDQEVKLKVDSFRERITSEAEDLVANFFPKKLLELDSFLKEPILNIHDLTQIHSDMNLPVPDPILLTNSHDGLDGPTYKKRRLDECEEAFQGTKVFVMPNGMLKSNQQLVDIIEKVKPEIRLLIEKCNTVKMWVQLLIPRIEDGNNFGVSIQEETVAELRTVESEAASYLDQISRYYITRAKLVSKIAKYPHVEDYRRTVTEIDEKEYISLRLIISELRNQYVTLHDMILKNIEKIKRPRSSNAETLY

Approximately 33 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.