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  3. PSMG3 蛋白, Human (His, Strep)

PSMG3 蛋白, Human (His, Strep)

目录号: HY-P701389
产品使用指南

PSMG3 蛋白充当伴侣并积极促进 20S 蛋白酶体的组装。它涉及与 PSMG1-PSMG2 异二聚体的潜在合作,以确保蛋白酶体的精确组装。PSMG3 蛋白, Human (His, Strep) 是重组的 PSMG3 蛋白,由 E. coli 表达,带有 N-Strep, N-6*His 标签。PSMG3 蛋白, Human (His, Strep) 全长 122 个氨基酸。

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描述

The PSMG3 protein acts as a chaperone and actively promotes the assembly of the 20S proteasome. It involves potential cooperation with the PSMG1-PSMG2 heterodimer to ensure precise assembly of the proteasome. PSMG3 Protein, Human (His, Strep) is the recombinant human-derived PSMG3 protein, expressed by E. coli , with N-Strep, N-6*His labeled tag. The total length of PSMG3 Protein, Human (His, Strep) is 122 a.a., .

研究背景

PSMG3(蛋白酶体组装伴侣 3)是一种对于 20S 蛋白酶体组装至关重要的伴侣蛋白,而 20S 蛋白酶体是细胞蛋白质降解机制的重要组成部分。PSMG3 可能与 PSMG1-PSMG2 异二聚体合作来协调蛋白酶体的正确组装。PSMG3 作为同二聚体存在,与 PSMG4 相互作用,并直接与 20S 蛋白酶体的 α 和 β 亚基结合。值得注意的是,它在半蛋白酶体形成之前解离,很可能在 POMP 招募时发生。这凸显了 PSMG3 在促进 20S 蛋白酶体精确组装中的作用,这是通过有效的蛋白质降解维持细胞稳态的关键过程。

种属

Human

表达系统

E. coli

标签

N-Strep;N-6*His

蛋白编号

Q9BT73 (M1-W122)

基因 ID

84262

蛋白结构
N-term
Strep-6*His
PSMG3 (M1-W122)
Accession # Q9BT73
C-term
同用名
PSMG3; Proteasome assembly chaperone 3; PAC-3; hPAC3
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PSMG3 Protein, Human (His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PSMG3 Protein, Human (His, Strep)
目录号:
HY-P701389
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