2. 重组蛋白
  3. Cytokines and Growth Factors
  4. Peptide Hormone & Neuropeptides
  5. PTH 蛋白, Human (GST)

PTH 蛋白, Human (GST)

目录号: HY-P73686
产品使用指南

PTH 蛋白或甲状旁腺激素通过溶解骨盐和抑制肾脏排泄来提高钙水平。它还刺激成骨细胞中的 [1-14C]-2-脱氧-D-葡萄糖转运和糖原合成，与 PTH1R 受体相互作用并与其 N 末端胞外结构域结合以产生这些作用。PTH 蛋白, Human (GST) 是重组的 PTH 蛋白，由 E. coli 表达，带有 N-GST 标签。

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描述

PTH Protein, or parathyroid hormone, raises calcium levels by dissolving bone salts and inhibiting kidney excretion. It also stimulates [1-14C]-2-deoxy-D-glucose transport and glycogen synthesis in osteoblastic cells, interacting with the PTH1R receptor and binding to its N-terminal extracellular domain for these effects. PTH Protein, Human (GST) is the recombinant human-derived PTH protein, expressed by E. coli , with N-GST labeled tag.
研究背景

PTH（甲状旁腺激素）通过促进骨盐溶解并抑制肾脏排泄来提高钙水平。此外，它还能刺激 [1-14C]-2-脱氧-D-葡萄糖 (2DG) 转运并促进成骨细胞中的糖原合成。PTH 通过与 PTH1R 受体相互作用，特别是与受体的 N 末端胞外结构域结合来实现这些效果。
种属

Human
表达系统

E. coli
标签

N-GST
蛋白编号

P01270 (S32-F65)
基因 ID
蛋白结构
N-term
GST
PTH (S32-F65)
Accession # P01270
C-term
同用名
Parathyroid hormone; PTH; Parathormone; Parathyrin
氨基酸序列

SVSEIQLMHNLGKHLNSMERVEWLRKKLQDVHNF

分子量

Approximately 30 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

PTH Protein, Human (GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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