PTH 蛋白, Human

PTH Protein, Human is a major regulator of mineral ion metabolism.

重组人甲状旁腺激素是内源性甲状旁腺激素分子的复制品。甲状旁腺激素通过作用于骨骼、肾脏和肠道来调节钙和磷的水平。在骨骼中，甲状旁腺激素通过刺激骨吸收来动员钙和磷进入循环。甲状旁腺激素刺激成骨细胞增加核因子 jB 配体受体激活剂 (RANKL) 的表达^[1]。

1.PTH is fully biologically active when compared to standards. The activity calculated by UMR106 cell/cAMP method corresponding to a specifc activity of 1.0×10⁴ U/mg.
2.Measured by its ability to induce cAMP accumulation in MC3T3‑E1 mouse preosteoblast cells. The ED₅₀ for this effect is 26.74 ng/mL, corresponding to a specific activity is 3.739×10⁴ U/mg.

• 
  Measured by its ability to induce cAMP accumulation in MC3T3-E1 mouse preosteoblast cells. The ED₅₀ for this effect is 26.74 ng/mL, corresponding to a specific activity is 3.739×10⁴ U/mg.

Human

E. coli

Tag Free

P01270 (S32-Q115)

5741  [NCBI]

SVSEIQLMHNLGKHLNSMERVEWLRKKLQDVHNFVALGAPLAPRDAGSQRPRKKEDNVLVESHEKSLGEADKADVNVLTKAKSQ

Approximately 9-15 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against 1.15 mg sodium citrate, sodium chloride 7.31 mg, 0.21 mg citric acid, 0.1117 mg EDTA-Na₂, 0.2 mg Tween 80 and 50 mg Mannitol or 10 mM HAc-NaAc, 150 mM NaCl, 5% Mannitol, pH 4.0 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Kim ES, et al. Recombinant Human Parathyroid Hormone (1-84): A Review in Hypoparathyroidism. Drugs. 2015 Jul;75(11):1293-303.  [Content Brief]