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  4. PTPN12 蛋白, Human (sf9, His-GST)

PTPN12 蛋白, Human (sf9, His-GST)

目录号: HY-P76558
产品使用指南 技术支持

PTPN12蛋白是一种多功能酶,可以使多种蛋白去磷酸化并调节细胞信号级联。据文献报道,PTPN12选择性地靶向ERBB2和PTK2B/PYK2等酪氨酸激酶来控制相关通路。PTPN12 蛋白, Human (sf9, His-GST) 是重组的 PTPN12 蛋白,由 Sf9 insect cells 表达,带有 N-GST, N-His 标签。PTPN12 蛋白, Human (sf9, His-GST) 全长 355 个氨基酸,分子量约为 ~64 kDa。

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描述

The PTPN12 protein is a multifunctional enzyme that can dephosphorylate a variety of proteins and regulate cell signaling cascades. According to literature, PTPN12 selectively targets tyrosine kinases such as ERBB2 and PTK2B/PYK2 to control related pathways. PTPN12 Protein, Human (sf9, His-GST) is the recombinant human-derived PTPN12 protein, expressed by Sf9 insect cells , with N-GST, N-His labeled tag. The total length of PTPN12 Protein, Human (sf9, His-GST) is 355 a.a., with molecular weight of ~64 kDa.

研究背景

正如文献中记载的那样,PTPN12 蛋白作为一种多功能酶,能够使一系列蛋白质去磷酸化,从而在各种细胞信号级联的调节中发挥着至关重要的作用。在其底物中,PTPN12 选择性地使细胞酪氨酸激酶(包括 ERBB2 和 PTK2B/PYK2)去磷酸化,对涉及这些激酶的信号通路发挥调节控制。PTPN12 的特异性通过其在特定酪氨酸残基(例如“Tyr-1112”、“Tyr-1196”和/或“Tyr-1248”)处使 ERBB2 去磷酸化的能力而凸显。PTPN12 复杂的去磷酸化活性强调了其在调节关键信号传导事件和微调酪氨酸激酶途径介导的细胞反应中的重要性。

种属

Human

表达系统

Sf9 insect cells

标签

N-GST;N-His

蛋白编号

Q05209 (M1-Q355)

基因 ID
蛋白结构
N-term
His-GST
PTPN12 (M1-Q355)
Accession # Q05209
C-term
同用名
Tyrosine-protein phosphatase non-receptor type 12; PTP-PEST; PTPG1
分子量

Approximately 64 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PTPN12 Protein, Human (sf9, His-GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PTPN12 Protein, Human (sf9, His-GST)
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HY-P76558
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