RAB7A 蛋白, Rat (N-His)

RAB7A is a dynamic small GTPase that coordinates endolysosomal trafficking by cycling between a GTP-bound state and a GDP-bound state. In its active state, RAB7A interacts with various effectors, affecting endosomal maturation, microtubule directed migration, and endosome-lysosomal trafficking. RAB7A Protein, Rat (N-His) is the recombinant rat-derived RAB7A, expressed by E. coli , with N-His labeled tag. ,

RAB7A 是一种小型 GTP 酶，在其活性 GTP 结合状态和非活性 GDP 结合状态之间经历动态循环，对内溶酶体运输发挥多方面的控制。在其活性状态下，RAB7A 与各种效应蛋白结合，通过复杂的蛋白质-蛋白质相互作用影响早期到晚期的内体成熟、微管引导的内体迁移以及内体-溶酶体运输。除了在内体运输中发挥核心作用外，RAB7A 还显着影响多种细胞和生理过程，包括生长因子介导的细胞信号传导、营养转运蛋白介导的营养吸收、神经元轴突中的神经营养蛋白运输和脂质代谢。此外，它还参与专门的内体膜运输事件，例如黑素体成熟、病原体诱导的吞噬体和自噬体。RAB7A 在吞噬体与溶酶体的成熟、酸化和融合中至关重要，这对于处理微生物病原体至关重要。它的参与延伸到微生物病原体的感染和生存，影响各种病毒的生命周期。此外，RAB7A 与 RAC1 协同调节破骨细胞中褶皱边界的形成，并在神经突生长信号传导、EGF-EGFR 复合物运输、ADRB2 通过脂肪吞噬刺激的脂肪分解、特定蛋白的外泌体释放、ACE2 的细胞表面表达以及潜在的作用中发挥作用。PRPH 神经元中间丝组装。

Rat

E. coli

N-His

P09527 (M1-C207)

29448

MTSRKKVLLKVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVMVDDRLVTMQIWDTAGQERFQSLGVAFYRGADCCVLVFDVTAPNTFKTLDSWRDEFLIQASPRDPENFPFVVLGNKIDLENRQVATKRAQAWCYSKNNIPYFETSAKEAINVEQAFQTIARNALKQETEVELYNEFPEPIKLDKNERAKASAESCSC

Approximately 23-25 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.