RACK1 蛋白, Human (His-MBP)

The RACK1 protein is a multifunctional scaffold protein that participates in multiple cellular processes by recruiting, assembling, and regulating signaling molecules. As part of the 40S ribosomal subunit, it contributes to translation repression and initiates the ribosome quality control (RQC) pathway by promoting ubiquitination of specific 40S ribosomal subunits. RACK1 Protein, Human (His-MBP) is the recombinant human-derived RACK1 protein, expressed by E. coli , with N-His, N-MBP labeled tag. The total length of RACK1 Protein, Human (His-MBP) is 317 a.a., with molecular weight of ~70 kDa.

RACK1 作为一种多功能支架蛋白，通过招募、组装和调节各种信号分子复杂地参与多种细胞过程。作为 40S 核糖体亚基的组成部分，它有助于翻译抑制，并且对于启动核糖体质量控制 (RQC) 途径至关重要，特别是通过促进 40S 核糖体亚基子集的泛素化。RACK1 结合并稳定活化的蛋白激酶 C (PKC)，从而增强 PKC 介导的磷酸化，并可能将其募集到核糖体中以实现 EIF6 的磷酸化。此外，RACK1 对 SRC 激酶（包括 SRC、LCK 和 YES1）发挥抑制作用，导致细胞周期停滞在 G0/G1 期。其调节作用延伸至多种细胞功能，例如调节整合素信号传导、促进细胞凋亡、调节各种受体的膜定位以及影响细胞迁移。此外，RACK1 还涉及病原体与宿主的相互作用，其与假结核耶尔森氏菌 yopK 的结合证明了这一点，从而抑制吞噬作用并促进细菌在宿主细胞内存活。

Human

E. coli

N-His;N-MBP

P63244 (M1-R317)

10399  [NCBI]

MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWNTLGVCKYTVQDESHSEWVSCVRFSPNSSNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGGDIINALCFSPNRYWLCAATGPSIKIWDLEGKIIVDELKQEVISTSSKAEPPQCTSLAWSADGQTLFAGYTDNLVRVWQVTIGTR

Approximately 70 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.