MEC/CCL28 蛋白, Human

MEC/CCL28 Protein, Human is a CC chemokine that is present in almost all mucosal tissues and acts as a unifying immunostimulant on mucosal surfaces. CCL28 can bind to CCR3 and CCR10 to mediate immune responses, viral infections, cancer, and antimicrobial effects. MEC/CCL28 Protein, Human is a recombinant human MEC/CCL28(S20-Y127) protein expressed by E. coli^[1][2].

CCL28，也称为粘膜相关上皮趋化因子 (MEC)、CCK1 和 SCYA28，是一种趋化因子。它在多种粘膜部位表达，包括唾液腺和乳腺、气管和结肠以及小肠。CCL28已被列为稳态淋巴细胞转运的重要组成趋化因子，可以与CCR3和CCR10结合。其中，CCL28可以趋化CCR10表达的CD4和CD8 T细胞群，以及CCR3表达的嗜酸性粒细胞迁移。然而，在肠粘膜中，很少有T细胞表达CCR10。相比之下，在 B 细胞群体中，CCR10 可以由 IgA 浆母细胞和 IgA 分泌细胞（即浆细胞）选择性表达，这在浆母细胞归巢至肠外效应位点中发挥关键作用^[1]。CCL28 在结肠中组成型表达，但其水平可以通过促炎细胞因子和某些细菌产物来增加，这些细菌产物在效应细胞募集到上皮损伤部位方面发挥作用。CCL28 可以作为粘膜表面的统一免疫刺激剂，并且参与表达 IgA 的细胞迁移至乳腺、唾液腺、肠道和其他粘膜组织。此外，CCL28 对革兰氏阴性和革兰氏阳性细菌以及真菌（如铜绿假单胞菌和肺炎克雷伯菌）表现出广谱抗菌活性。进一步的研究还表明，CCL28 C 末端带正电荷的氨基酸对该蛋白的抗菌活性有显着贡献，其特有的疏水性和两亲性也有助于其杀伤活性^[2] 。

CCL28 (0.1-100 ng/mL) 剂量依赖性增强内皮细胞趋化性，促进 HUVECs 的管状形成。CCL28 (10 ng/mL, 100 ng/mL；1 h) 磷酸化内皮细胞中的 ERK，但不磷酸化 p38、AKT、JNK^[6]。

1.Full biological activity determined by a chemotaxis bioassay using human lymphocytes is in a concentration range of 1.0-10.0 ng/ml.
2.Measured by its ability to chemoattract MDA-MB-231 cells. The ED₅₀ for this effect is 28.27 ng/mL, corresponding to a specific activity is 35373.187 U/mg.

Human

E. coli

Tag Free

Q9NRJ3-1 (S20-Y127)

56477  [NCBI]

SEAILPIASSCCTEVSHHISRRLLERVNMCRIQRADGDCDLAAVILHVKRRRICVSPHNHTVKQWMKVQAAKKNGKGNVCHRKKHHGKRNSNRAHQGKHETYGHKTPY

Approximately 12.4 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against 20 mM PB, pH 7.4, 130 mM NaCl or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Hiroyuki Ogawa, et al. Regulated production of the chemokine CCL28 in human colon epithelium. Am J Physiol Gastrointest Liver Physiol. 2004 Nov;287(5):G1062-9.  [Content Brief]

  [2]. Meurens F, et al. Expression of mucosal chemokines TECK/CCL25 and MEC/CCL28 during fetal development of the ovine mucosal immune system. Immunology. 2007 Apr;120(4):544-55.  [Content Brief]

  [3]. Teena Mohan, et al. CCL28 chemokine: An anchoring point bridging innate and adaptive immunity. Int Immunopharmacol. 2017 Oct;51:165-170.  [Content Brief]

  [4]. Chan Sun, et al. Chemokine CCL28 induces apoptosis of decidual stromal cells via binding CCR3/CCR10 in human spontaneous abortion. Mol Hum Reprod. 2013 Oct;19(10):676-86.  [Content Brief]

  [5]. Malin Hansson, et al. CCL28 is increased in human Helicobacter pylori-induced gastritis and mediates recruitment of gastric immunoglobulin A-secreting cells. Infect Immun. 2008 Jul;76(7):3304-11.  [Content Brief]

  [6]. Chen Z, et al. Characterising the expression and function of CCL28 and its corresponding receptor, CCR10, in RA pathogenesis. Ann Rheum Dis. 2015 Oct;74(10):1898-906.  [Content Brief]