IL-3 蛋白, Mouse (His)

IL-3 Protein, Mouse (His) is a pleiotropic cytokine containing 135 amino acids, which functions via specific cell surface receptors to stimulate the proliferation, differentiation and survival of haematopoietic cell lines.

白细胞介素-3 (IL-3) 是属于造血生长因子家族的糖蛋白，在临床前体外和体内研究中已表现出多谱系活性。重组人白细胞介素 3 (rhIL-3) 通过重组人粒细胞集落刺激因子 (rhG-CSF) 增强外周血祖细胞的动员^[1]。人白细胞介素 3 (hIL-3) 是一种多能造血细胞因子，由有丝分裂原和抗原激活的角质形成细胞、T 淋巴细胞、肥大细胞、NK 细胞、单核细胞和内皮细胞产生。造血祖细胞在hIL-3蛋白的帮助下增殖并分化为成熟红细胞、肥大细胞、巨核细胞和粒细胞。hIL-3 蛋白的潜在用途已在骨髓移植、血液恶性肿瘤、血细胞减少症、再生障碍性贫血和各种类型的癌症等各种临床应用中得到广泛测试^[2]。

Measured in a cell proliferation assay using M-NFS-60 cells. The ED₅₀ for this effect is 77.96 pg/mL, corresponding to a specific activity is 1.282×10⁷ units/mg.

Mouse

E. coli

N-6*His

P01586 (D33-C166)

16187  [NCBI]

DTHRLTRTLNCSSIVKEIIGKLPEPELKTDDEGPSLRNKSFRRVNLSKFVESQGEVDPEDRYVIKSNLQKLNCCLPTSANDSALPGVFIRDLDDFRKKLRFYMVHLNDLETVLTSRPPQPASGSVSPNRGTVEC

Approximately 17 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 6.5, 8% trehalose.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Huhn RD, et al. Recombinant human interleukin-3 (rhIL-3) enhances the mobilization of peripheral blood progenitor cells by recombinant human granulocyte colony-stimulating factor (rhG-CSF) in normal volunteers. Exp Hematol. 1996 Jun;24(7):839-47.  [Content Brief]

  [2]. Dagar VK, et al. Combined effect of gene dosage and process optimization strategies on high-level production of recombinant human interleukin-3 (hIL-3) in Pichia pastoris fed-batch culture. Int J Biol Macromol. 2018 Mar;108:999-1009.  [Content Brief]