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  2. IL-3 蛋白, Mouse (His)

IL-3 蛋白, Mouse (His)

目录号: HY-P7062A
COA 产品使用指南

IL-3 蛋白, Mouse (His)是一种含有 135 个氨基酸的多效细胞因子,通过特异性细胞表面受体起作用,刺激造血细胞系增殖,分化和存活。

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规格 价格 是否有货 数量
100 μg ¥6300 In-stock
> 100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

IL-3 Protein, Mouse (His) is a pleiotropic cytokine containing 135 amino acids, which functions via specific cell surface receptors to stimulate the proliferation, differentiation and survival of haematopoietic cell lines.

研究背景

白细胞介素-3 (IL-3) 是属于造血生长因子家族的糖蛋白,在临床前体外和体内研究中已表现出多谱系活性。重组人白细胞介素 3 (rhIL-3) 通过重组人粒细胞集落刺激因子 (rhG-CSF) 增强外周血祖细胞的动员[1]。人白细胞介素 3 (hIL-3) 是一种多能造血细胞因子,由有丝分裂原和抗原激活的角质形成细胞、T 淋巴细胞、肥大细胞、NK 细胞、单核细胞和内皮细胞产生。造血祖细胞在hIL-3蛋白的帮助下增殖并分化为成熟红细胞、肥大细胞、巨核细胞和粒细胞。hIL-3 蛋白的潜在用途已在骨髓移植、血液恶性肿瘤、血细胞减少症、再生障碍性贫血和各种类型的癌症等各种临床应用中得到广泛测试[2]

生物活性

Measured in a cell proliferation assay using M-NFS-60 cells. The ED50 for this effect is 77.96 pg/mL, corresponding to a specific activity is 1.282×107 units/mg.

种属

Mouse

表达系统

E. coli

标签

N-6*His

蛋白编号

P01586 (D33-C166)

基因 ID
蛋白结构
N-term
IL-3 (D33-C166)
Accession # P01586
C-term
中文名
重组鼠IL-3蛋白
同用名
rMuIL-3; Hematopoietic growth factor; Mast cell growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor
氨基酸序列

DTHRLTRTLNCSSIVKEIIGKLPEPELKTDDEGPSLRNKSFRRVNLSKFVESQGEVDPEDRYVIKSNLQKLNCCLPTSANDSALPGVFIRDLDDFRKKLRFYMVHLNDLETVLTSRPPQPASGSVSPNRGTVEC

分子量

Approximately 17 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末

组分

Lyophilized from a 0.22 μm filtered solution of PBS, pH 6.5, 8% trehalose.

内毒素含量

<0.2 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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