1. 重组蛋白
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  3. REG-1 beta/REG1B 蛋白, Human (HEK293, His)

REG-1 beta/REG1B 蛋白, Human (HEK293, His)

目录号: HY-P76565
COA 产品使用指南

REG1B 蛋白可能抑制自发碳酸钙沉淀,并可能与大脑中的神经元萌芽有关。它还有助于大脑和胰腺组织的再生。REG-1 beta/REG1B 蛋白, Human (HEK293, His) 是重组的 REG-1 beta/REG1B 蛋白,由 HEK293 表达,带有 C-His 标签。REG-1 beta/REG1B 蛋白, Human (HEK293, His) 全长 144 个氨基酸,分子量约为 ~19-20 kDa。

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5 μg ¥550 In-stock
10 μg ¥950 In-stock
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100 μg ¥4500 In-stock
500 μg ¥12600 In-stock
1 mg ¥21400 In-stock
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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

REG1B Protein potentially inhibits spontaneous calcium carbonate precipitation and may be associated with neuronal sprouting in the brain. It also contributes to the regeneration of brain and pancreas tissues. REG-1 beta/REG1B Protein, Human (HEK293, His) is the recombinant human-derived REG-1 beta/REG1B protein, expressed by HEK293 , with C-His labeled tag. The total length of REG-1 beta/REG1B Protein, Human (HEK293, His) is 144 a.a., with molecular weight of ~19-20 kDa.

研究背景

REG1B 蛋白可能充当自发碳酸钙沉淀的抑制剂,并可能与大脑中神经元萌芽等过程以及参与大脑和胰腺组织的再生有关。

生物活性

Measured in a cell proliferation assay using RT4‑D6P2T rat schwannoma cells. The ED50 for this effect is 0.657 μg/mL, corresponding to a specific activity is 1.522×10^3 units/mg.

种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

P48304 (Q23-N166)

基因 ID
蛋白结构
N-term
REG1B (Q23-N166)
Accession # P48304
His
C-term
同用名
Lithostathine-1-beta; PSP-2; REG-1-beta; PSPS2; REGL
氨基酸序列

QESQTELPNPRISCPEGTNAYRSYCYYFNEDPETWVDADLYCQNMNSGNLVSVLTQAEGAFVASLIKESSTDDSNVWIGLHDPKKNRRWHWSSGSLVSYKSWDTGSPSSANAGYCASLTSCSGFKKWKDESCEKKFSFVCKFKN

分子量

Approximately 19-20 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

REG-1 beta/REG1B Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
REG-1 beta/REG1B Protein, Human (HEK293, His)
目录号:
HY-P76565
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