RET 蛋白, Human (HEK293, His)

RET protein is a receptor tyrosine protein kinase that plays a key role in cell proliferation, neuronal navigation, and differentiation upon binding to glial cell-derived neurotrophic factor family ligands. It regulates the delicate balance between cell death and survival, influences positional information, and is critical for intestinal organogenesis, enteric nervous system development, renal organogenesis, and Peyer's patch formation. RET Protein, Human (HEK293, His) is the recombinant human-derived RET protein, expressed by HEK293 , with N-His labeled tag.

RET 蛋白是一种受体酪氨酸蛋白激酶，在与神经胶质细胞源性神经营养因子家族配体结合后，在细胞增殖、神经元导航、迁移和分化等多种细胞过程中发挥着至关重要的作用。它磷酸化 PTK2/FAK1 并调节细胞死亡和存活以及位置信息之间的微妙平衡。RET 对于肠器官发生过程中的分子协调至关重要，它参与肠神经系统的发育、肾器官发生，并促进肠道相关淋巴组织中派尔氏斑样结构的形成。此外，RET 通过交感神经元中的半胱天冬酶裂解介导调节细胞粘附，并以整合素依赖性方式促进细胞迁移。作为依赖性受体，它在生长激素细胞中缺乏配体 GDNF 的情况下触发细胞凋亡，但在其存在的情况下促进存活并下调生长激素的产生。RET 是多种疾病的关键介质，特别是以异常整合素调节的细胞迁移为特征的神经内分泌癌。此外，它通过与 GFRAL 相互作用介导 GDF15 诱导的脑干细胞信号传导，从而抑制食物摄入并激活 MAPK 和 AKT 信号通路。值得注意的是，与同一复合物中的异构体 2 相比，与 GFRAL 复合的异构体 1 诱导 MAPK 信号通路更高的激活。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

N-His

P07949-1 (L29-R635)

5979  [NCBI]

LYFSRDAYWEKLYVDQAAGTPLLYVHALRDAPEEVPSFRLGQHLYGTYRTRLHENNWICIQEDTGLLYLNRSLDHSSWEKLSVRNRGFPLLTVYLKVFLSPTSLREGECQWPGCARVYFSFFNTSFPACSSLKPRELCFPETRPSFRIRENRPPGTFHQFRLLPVQFLCPNISVAYRLLEGEGLPFRCAPDSLEVSTRWALDREQREKYELVAVCTVHAGAREEVVMVPFPVTVYDEDDSAPTFPAGVDTASAVVEFKRKEDTVVATLRVFDADVVPASGELVRRYTSTLLPGDTWAQQTFRVEHWPNETSVQANGSFVRATVHDYRLVLNRNLSISENRTMQLAVLVNDSDFQGPGAGVLLLHFNVSVLPVSLHLPSTYSLSVSRRARRFAQIGKVCVENCQAFSGINVQYKLHSSGANCSTLGVVTSAEDTSGILFVNDTKALRRPKCAELHYMVVATDQQTSRQAQAQLLVTVEGSYVAEEAGCPLSCAVSKRRLECEECGGLGSPTGRCEWRQGDGKGITRNFSTCSPSTKTCPDGHCDVVETQDINICPQDCLRGSIVGGHEPGEPRGIKAGYGTCNCFPEEEKCFCEPEDIQDPLCDELCR

110-120 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.