RET 蛋白, Mouse (HEK293, His)

RET protein is an important receptor tyrosine protein kinase that drives a variety of cellular processes such as proliferation, migration, and differentiation when bound to GDNF ligand. It coordinates organogenesis, shapes the enteric nervous system and promotes kidney development. RET Protein, Mouse (HEK293, His) is the recombinant mouse-derived RET protein, expressed by HEK293 , with C-6*His labeled tag and F174S mutation. The total length of RET Protein, Mouse (HEK293, His) is 609 a.a.

RET 蛋白是一种受体酪氨酸蛋白激酶，当它与胶质细胞源性神经营养因子家族配体结合时，在细胞增殖、神经元导航、细胞迁移和细胞分化等各种细胞过程中发挥着至关重要的作用。它磷酸化 PTK2/FAK1 并调节细胞死亡和存活之间的平衡以及位置信息。在肠道器官发生过程中，RET蛋白对于协调分子机制至关重要，并参与胚胎生命中肠神经系统和肾器官发生的发育。此外，它还能促进派尔氏斑状结构的形成，这是肠道相关淋巴组织的重要组成部分。RET 蛋白通过交感神经元中 caspase 的裂解来调节细胞粘附，并以整合素依赖性方式促进细胞迁移，涉及 ITGB1 和 ITGB3 等蛋白。此外，RET 蛋白与神经嵴的发育有关。在没有配体的情况下，它表现出通过受体细胞内 caspase 裂解触发细胞凋亡的活性。此外，RET蛋白充当依赖性受体，当配体GDNF存在时促进生长激素细胞的存活并下调生长激素的产生，但在不存在GDNF时诱导细胞凋亡。它还调节伤害感受器的存活和大小，并触发快速适应的机械感受器的分化。在各种疾病中，例如神经内分泌癌，RET 蛋白通过调节由异常整合素调节的细胞迁移来充当介质。此外，RET 蛋白与脑干中的 GDF15 受体 GFRAL 相互作用，介导 GDF15 诱导的细胞信号传导，从而抑制食物摄入。此外，RET 蛋白激活 MAPK 和 AKT 信号通路。

Measured by its binding ability in a functional ELISA. Immobilized GDNF-GFR alpha-1 complex using 4 ng/mL rmGDNF (HY-P7359) and 1 µg/mL rmGDNFR-alpha-1(HY-P76360), can bind Recombinant biotinylated Mouse RET. The ED₅₀ for this effect is 2.282 μg/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized GDNF-GFR alpha-1 complex using 4 ng/mL rmGDNF (HY-P7359) and 1 µg/mL rmGDNFR-alpha-1 (HY-P76360), can bind Recombinant biotinylated Mouse RET. The ED₅₀ for this effect is 2.282 μg/mL.

Mouse

HEK293

C-6*His

P35546 (L29-R637, F174S)

19713

LYFSRDAYWERLYVDQPAGTPLLYVHALRDAPGEVPSFRLGQHLYGVYRTRLHENDWIRINETTGLLYLNQSLDHSSWEQLSIRNGGFPLLTIFLQVFLGSTAQREGECHWPGCTRVYFSFINDTFPNCSSFKAQDLCIPETAVSSRVRENRPPGTFYHFHMLPVQFLCPNISVKYSLLGGDSLPFRCDPDCLEVSTRWALDRELREKYVLEALCIVAGPGANKETVTLSFPVTVYDEDDSAPTFSGGVGTASAVVEFKRKEGTVVATLQVFDADVVPASGELVRRYTNTLLSGDSWAQQTFRVEHSPIETLVQVNNNSVRATMHNYKLILNRSLSISESRVLQLAVLVNDSDFQGPGAGGILVLHFNVSVLPVTLNLPRAYSFPVNKRARRYAQIGKVCVENCQEFSGVSIQYKLQPSSINCTALGVVTSPEDTSGTLFVNDTEALRRPECTKLQYTVVATDRQTRRQTQASLVVTVEGTSITEEVGCPKSCAVNKRRPECEECGGLGSPTGRCEWRQGDGKGITRNFSTCSPSTRTCPDGHCDAVESRDANICPQDCLRADIVGGHERGERQGIKAGYGICNCFPDEKKCFCEPEDSQGPLCDALCR

Approximately 92-125 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<0.1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.