RhoA 蛋白, Human (sf9, His)

The RhoA protein is a critical small GTPase that dynamically regulates multiple cellular responses by cycling between an active GTP-bound state and an inactive GDP-bound state. It mainly affects cytoskeletal organization, interacts with various effectors, and affects cytoskeletal dynamics, cell migration, and cell cycle progression. RhoA Protein, Human (sf9, His) is the recombinant human-derived RhoA protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of RhoA Protein, Human (sf9, His) is 193 a.a., with molecular weight of ~28 kDa.

RhoA 蛋白是一种小型 GTP 酶，对于协调不同的细胞反应、在活跃的 GTP 结合状态和不活跃的 GDP 结合状态之间循环至关重要。RhoA 的活性形式主要与细胞骨架的组织相关，可与各种效应蛋白结合，影响细胞过程，例如细胞骨架动力学、细胞迁移和细胞周期进程。它在调节将质膜受体连接到粘着斑和肌动蛋白应力纤维组装的信号转导途径中发挥着关键作用。此外，RhoA 对于细胞周期胞质分裂期间肌球蛋白收缩环形成所需的微管依赖性信号是不可或缺的，有助于卵裂沟的形成。RhoA 对于角质细胞间粘附的顶端连接形成至关重要，还参与 MEMO1-RHOA-DIAPH1 信号通路，影响 ERBB2 依赖性微管稳定性。它进一步调节钾通道活性，调节鸟嘌呤核苷酸交换因子，并在微生物感染过程中充当鼠疫耶尔森氏菌 yopT 半胱氨酸肽酶的靶标。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-His

P61586 (M1-L193)

387  [NCBI]

Approximately 28 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10% Glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.