RIZ1 蛋白, Human (His)

RIZ1 Protein, an S-adenosyl-L-methionine-dependent histone methyltransferase, specifically methylates 'Lys-9' of histone H3. Additionally, it acts as a DNA-binding transcription factor, showing affinity for the MTE within the HMOX1 gene. Implicated as a potential activator, RIZ1 suggests a regulatory role in HMOX1 gene expression beyond histone modification. RIZ1 Protein, Human (His) is the recombinant human-derived RIZ1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of RIZ1 Protein, Human (His) is 200 a.a., with molecular weight of ~27 kDa.

RIZ1 蛋白作为 S-腺苷-L-甲硫氨酸依赖性组蛋白甲基转移酶，表现出对组蛋白 H3 的“Lys-9”甲基化的特异性。除了其组蛋白修饰作用之外，RIZ1 还可以充当 DNA 结合转录因子，与 HMOX1（血红素加氧酶 1）基因内的巨噬细胞特异性 TPA 响应元件 (MTE) 具有亲和力。在这种情况下，它被认为是 HMOX1 的潜在转录激活剂，表明该基因的表达具有调节作用。

Human

E. coli

N-6*His

Q13029 (M1-A200)

7799  [NCBI]

MNQNTTEPVAATETLAEVPEHVLRGLPEEVRLFPSAVDKTRIGVWATKPILKGKKFGPFVGDKKKRSQVKNNVYMWEVYYPNLGWMCIDATDPEKGNWLRYVNWACSGEEQNLFPLEINRAIYYKTLKPIAPGEELLVWYNGEDNPEIAAAIEEERASARSKRSSPKSRKGKKKSQENKNKGNKIQDIQLKTSEPDFTSA

Approximately 27 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.