1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. Rnase 1 蛋白, Human (HEK293, His)

Rnase 1 蛋白, Human (HEK293, His)

目录号: HY-P71089
COA 产品使用指南

RNase 1 蛋白作为核酸内切酶发挥作用,能够催化特定于嘧啶核苷酸 3' 侧的 RNA 裂解。这种酶活性扩展到单链和双链 RNA,证明了其在 RNA 底物识别和加工方面的多功能性。Rnase 1 蛋白, Human (HEK293, His) 是重组的 核糖核酸酶 1 蛋白,由 HEK293 表达,带有 C-6*His 标签。Rnase 1 蛋白, Human (HEK293, His) 全长 128 个氨基酸,分子量为 21-28 kDa。

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规格 价格 是否有货 数量
10 μg ¥1700 In-stock
50 μg ¥5100 询价
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The RNase 1 protein functions as an endonuclease capable of catalyzing RNA cleavage specific to the 3' side of pyrimidine nucleotides. This enzymatic activity extends to single- and double-stranded RNA, demonstrating its versatility in RNA substrate recognition and processing. Rnase 1 Protein, Human (HEK293, His) is the recombinant human-derived Rnase 1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Rnase 1 Protein, Human (HEK293, His) is 128 a.a., with molecular weight of 21-28 kDa.

研究背景

RNase 1 蛋白是一种核酸内切酶,在催化 RNA 分子裂解中发挥着至关重要的作用,特别针对嘧啶核苷酸的 3' 侧。这种酶活性不限于特定的 RNA 构象,因为已知 RNase 1 对单链和双链 RNA 都起作用。通过促进 RNA 分子的精确切割,RNase 1 有助于细胞过程中 RNA 的调节和周转。它靶向富含嘧啶区域的能力表明了广泛的潜在底物,突出了它在 RNA 代谢和细胞稳态中的重要性。(

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

P07998 (K29-T156)

基因 ID
蛋白结构
N-term
Rnase 1 (K29-T156)
Accession # P07998
6*His
C-term
同用名
Ribonuclease Pancreatic; HP-Rnase; RIB-1; RNase UpI-1; Ribonuclease 1; RNase 1; Ribonuclease A; RNase A; RNASE1; RIB1; RNS1
氨基酸序列

KESRAKKFQRQHMDSDSSPSSSSTYCNQMMRRRNMTQGRCKPVNTFVHEPLVDVQNVCFQEKVTCKNGQGNCYKSNSSMHITDCRLTNGSRYPNCAYRTSPKERHIIVACEGSPYVPVHFDASVEDST

分子量

21-28 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 10% Glycerol, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

Rnase 1 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Rnase 1 Protein, Human (HEK293, His)
目录号:
HY-P71089
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