ROBO1 蛋白, Rat (HEK293, His)

The ROBO1 protein is a receptor for SLIT1 and SLIT2 and critically mediates cellular responses during migration and axonal navigation. Interaction with FLRT3 promotes axonal attraction to NTN1-expressing cells. ROBO1 Protein, Rat (HEK293, His) is the recombinant rat-derived ROBO1 protein, expressed by HEK293 , with C-10*His labeled tag. The total length of ROBO1 Protein, Rat (HEK293, His) is 542 a.a., .

ROBO1 蛋白作为 SLIT1 和 SLIT2 的受体，在细胞迁移过程中介导细胞对分子引导线索的反应至关重要，包括神经管腹侧中线的轴突导航以及神经元发育过程中轴突向不同区域的投射。它与 FLRT3 胞内结构域的相互作用促进轴突对表达 NTN1 的细胞的吸引。在轴突生长锥中，SLIT2 对 NTN1 吸引作用的抑制可能需要形成 ROBO1-DCC 复合物。此外，ROBO1 通过与 MYO9B 相互作用来调节细胞迁移，抑制 MYO9B 介导的 RHOA GTP 酶活性刺激，从而导致活性 GTP 结合的 RHOA 水平升高。ROBO1 在肺部发育中具有潜在作用。作为同二聚体存在，二聚化由胞外结构域促进，并且不依赖于 SLIT 配体。ROBO1 蛋白与 SLIT1、SLIT2、FLRT3 和 MYO9B 相互作用，展示了其在细胞信号传导通路中的多种参与。

Measured in a cell proliferation assay using HUVEC cells. The ED₅₀ for this effect is typically 48.92 ng/mL, corresponding to a specific activity is 2.04×10⁴ units/mg.

Rat

HEK293

C-10*His

O55005 (K19-I560)

58946

KKHLLLAQLIPDPEDLERGNDNGTPAPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESKVADVTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTFGWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTVTNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLTLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLI

Approximately 70-95 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.