RPE 蛋白, Mouse (HEK293, His)

RPE proteins play a key role in cellular metabolism by catalyzing the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate. This enzyme activity is a key step in the non-oxidative phase of the pentose phosphate pathway and promotes the interconversion of pentose phosphates. RPE Protein, Mouse (HEK293, His) is the recombinant mouse-derived RPE protein, expressed by HEK293 , with C-His labeled tag. The total length of RPE Protein, Mouse (HEK293, His) is 228 a.a., with molecular weight of ~27 kDa.

5-磷酸核酮糖差向异构酶 (RPE) 是一种催化 D-核酮糖 5-磷酸可逆差向异构化为 D-木酮糖 5-磷酸的酶。这种酶活性是戊糖磷酸途径非氧化阶段的关键步骤，戊糖磷酸途径是糖相互转化和戊糖磷酸生成所必需的代谢途径。RPE 将核酮糖 5-磷酸可逆转化为木酮糖 5-磷酸，有助于合成各种重要的细胞成分，包括核苷酸和辅酶。在 RPE 等酶的促进下，磷酸戊糖途径的复杂调节确保了用于核苷酸生物合成的 5-磷酸核糖和用于细胞内氧化还原反应的 NADPH 的平衡供应。了解 RPE 的功能有助于深入了解糖代谢的调节及其在细胞过程中的重要性。参考资料：所提供的信息基于截至 2022 年 1 月的一般知识，未引用具体参考资料。

Mouse

HEK293

C-His

Q8VEE0 (M1-R228)

66646  [NCBI]

MASGCKIGPSILNSDLANLGAECLRMLDSGADYLHLDVMDGHFVPNITFGHPVVESLRKQLGQDPFFDMHMMVSRPEQWVKPMAVAGANQYTFHLEATENPGALIKDIRENGMKVGLAIKPGTTVEYLAPWANQIDMALVMTVEPGFGGQKFMEDMMPKVHWLRTQFPTLDIEVDGGVGPDTVQKCAEAGANMIVSGSAIMRSDDPRAVINLLRNVCSEAAQKRSLDR

Approximately 27 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.